General Information
|
|
Product
|
PDE4A4 Antibody BIOTIN-Conjugated
|
Description
|
BIOTIN-Conjugated cAMP specific phosphodiesterase 4A variant pde46 Antibody
|
Verified Applications
|
CM, ELISA, ICC/IF, IHC, IP, WB
|
Host
|
Rabbit
|
Species Cross Reactivity
|
Human, Mouse, Rabbit
|
Immunogen
|
N-terminus PDE4A4 synthetic cyclic peptide |
Specificity |
Does not cross react with PDE4A1, PDE4A5 or PDE4A8 proteins. Does not cross react with other PDE family members such as PDE4B, PDE4C and PDE4D. |
Accession #
|
GenBank: EAW74861.1
|
Physical Properties
|
|
Quantity
|
100 µg
|
Volume
|
200 µl
|
Concentration
|
1.00-1.25 µg/µl in antibody stabilization buffer
|
Form
|
BIOTIN-Conjugated
|
Clonality
|
Polyclonal
|
Storage
|
-20⁰C for long term storage
|
Application Protocol
|
Confocal Microscopy
|
1:100
|
DB
|
1:10,000
|
ELISA
|
1:10,000
|
Immunocytochemistry
|
1:100
|
Immunofluorescence
|
1:100
|
Immunohistochemistry
|
1:100
|
Immunoprecipitation
|
1:200
|
Western Blot
|
1:500
|
Protein
|
Uniprot #
|
P27815
|
Overview
|
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
|
Molecular Function
|
Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
|
Subcellular Location
|
Isoform 1: Cytoplasm › perinuclear region, Isoform 2: Cytoplasm, Cell projection, Isoform 4: Membrane; Peripheral membrane protein Isoform 6 : Cytoplasm › perinuclear region Isoform 7 : Cytoplasm. Membrane
|
Expression
|
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
|
Structure
|
Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.
|
Alternative Nomenclature
|
DPDE2 antibody
PDE4A antibody
Phosphodiesterase 4 antibody
Phosphodiesterase 4A antibody
Phosphodiesterse Type 4A4 antibody
|
|
|