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General Information
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Product
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Phospho FGFR4 Antibody BIOTIN-Conjugated
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Description
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BIOTIN-Conjugated Phosphorylated fibroblast growth factor receptor 4 variant Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 530-580 on human Fibroblast growth factor receptor 4 variant.
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Accession #
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GenBank: AAK51435.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.55-0.75 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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P22455
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Overview
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Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
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Molecular Function
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ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity
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Subcellular Location
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Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum
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Expression
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Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
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Structure
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Monomer. Homodimer after ligand binding. Interacts with FGF1, FGF2, FGF4, FGF6, FGF8, FGF9, FGF16, FGF17, FGF18, FGF19, FGF21 and FGF23 (in vitro). Binding affinity for FGF family members is enhanced by interactions between FGFs and heparan sulfate proteoglycans. Interacts with KLB; this strongly increases the affinity for FGF19 and FGF23. Affinity for FGF19 is strongly increased by KLB and sulfated glycosaminoglycans. KLB and KL both interact with the core-glycosylated FGFR4 in the endoplasmic reticulum and promote its degradation, so that only FGF4 with fully mature N-glycans is expressed at the cell surface. Identified in a complex with NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43, and CTTN. Interacts with MMP14 and HIP1.
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Alternative Nomenclature
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CD334 antigen antibody
fc13h 10 antibody
FGFR-4 antibody
Fibroblast growth factor receptor 4 antibody
Hydroxyaryl protein kinase antibody
JTK2 antibody
TKF antibody
Tyrosine kinase related to fibroblast growth factor receptor antibody
Tyrosylprotein kinase antibody
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Phospho-FGFR4 Blocking Peptide
Phospho-FGFR4 Antibody
Phospho-FGFR4 Antibody FITC
Phospho-FGFR4 Positive Control
