PMS2 Antibody BIOTIN

Product no.: PMS2.201-BIOTIN
$350.00


General Information

 

 

Product

 

PMS2 Antibody BIOTIN-Conjugated

Description

BIOTIN-Conjugated Mismatch Repair Endonuclease PMS2 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 220-270 on human Mismatch Repair endonuclease PMS2 protein.

Accession #

NCBINP_001308943


Physical Properties

 

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

BIOTIN-Conjugated

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20C for long term storage


Application Protocol

Dot Blot

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Immunohistochemistry 1:50-1:200
Immunoprecipitation 1:50-1:200

Western Blot

1:500-1:1,000


Protein

 

Uniprot #

 

P54278

Overview

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Molecular Function

ATPase activity, ATP binding, DNA binding, endonuclease activity, single base insertion or deletion binding

Structure

Heterodimer of PMS2 and MLH1 (MutL alpha). Forms a ternary complex with MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MTMR15/FAN1.

Alternative Nomenclature

DNA mismatch repair gene homologue antibody

DNA mismatch repair protein PMS2 antibody

HNPCC4 antibody

PMS1 protein homolog 2 antibody

PMS2 antibody

PMS2CL antibody

PMSL2 antibody

Postmeiotic segregation increased, S. cerevisiae, 2 antibody

   

Protein information supplied by UniProt

Accessories

Product Note Status Price
PMS2 Blocking Peptide PMS2 Blocking Peptide
P-PMS2.201 $145.00 *
PMS2 Antibody PMS2 Antibody
PMS2-201AP $265.00 *
PMS2 Antibody FITC PMS2 Antibody FITC
PMS2.201-FITC $350.00 *
PMS2 Positive Control PMS2 Positive Control
PC-PMS2 $185.00 *
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