Prominin 2 Antibody

Product no.: PROM-201AP

$285.00

Description
Ratings (1)

General Information
Product	Prominin 2 Antibody
Verified Applications	CM, ELISA, ICC/IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to Human Prominin 2 amino acids 315-338. Sequence: RTLELGADFSQVPSVDHVLHQLKG
Accession #	NCBI: NP_653308.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100
DB	1:2,000
ELISA	1:2,000
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q8N271
Molecular Function	Cholesterol binding
Subcellular Location	Apical cell membrane, multi-pass membrane protein (Note: Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells).
Expression	Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
Alternative Nomenclature	MGC138714 antibody PROM 2 antibody Prominin like protein 2 antibody Prominin related protein antibody Prominin-2 antibody

Protein information supplied by UniProt

Customer ratings for Prominin 2 Antibody

Number of ratings: 1
Average rating: 5

Excellent
from Anonymous on 11/24/2015

Immunocytochemistry/Immunofluorescence
BSA as blocking agent for 10 minutes at room temperature

Rat Cell (epithelium) Paraformaldehyde
1:500 Primary antibody dilution for 16 hours.
4% PFA used diluted in 0.1 M Phosphate Buffer.

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