Von Hippel Lindau Antibody

General Information

Product

Von Hippel Lindau Antibody

Description

Affinity Purified Von Hippel-Lindau disease tumor suppressor Antibody

Verified Applications

ELISA, IHC, IP, WB

Immunogen

Synthetic peptide taken within amino acid region 130-200 on human Von Hippel-Lindau disease tumor suppressor protein.

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P40337

Overview

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

Molecular Function

Enzyme binding, Transcription factor binding, Ubiquitin protein ligase activity,

Subcellular Location

Cytoplasm, Peripheral membrane protein, nucleus

Expression

Expressed in the adult and fetal brain and kidney.

Structure

Belongs to the VHL family.

Alternative Nomenclature

Elongin binding protein antibody

G7 protein antibody

HRCA 1 antibody

Protein G7 antibody

pVHL antibody

RCA 1 antibody

VHL 1 antibody

VHLH antibody

Von Hippel Lindau disease tumor suppressor antibody

von Hippel Lindau syndrome antibody

von Hippel Lindau tumor suppressor antibody

Von Hippel Lindau tumor suppressor, E3 ubiquitin protein ligase antibody