RIPK1 Antibody

Product no.: RIPK1-101AP
$295.00
$265.00


1_16

General Information

 

 

Product

 

RIPK1 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 1

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within aino acid region 620-670 on human Receptor-interacting Serine/Threonine-protein kinase 1 protein.

Accession #

NCBINP_003795.2


Physical Properties

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20C for long term storage


Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500


Protein

 

Uniprot #

 

Q13546

Overview

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Molecular Function

ATP binding; Death domain binding; Identical protein binding; Protein complex binding; Protein kinase activity; protein serine/threonine kinase activity; Ubiquitin protein ligase binding

Subcellular Location

Cytoplasm

Structure

Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif). Upon TNF-induced necrosis, the RIPK1-RIPK3 dimer further interacts with PGAM5 and MLKL; the formation of this complex leads to PGAM5 phosphorylation and increase in PGAM5 phosphatase activity. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1 (By similarity). Interacts with ZBP1 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK3 and MLKL. Interacts (via kinase domain) with DAB2IP (via Ras-GAP domain); the interaction occurs in a TNF-alpha-dependent manner. Interacts with ARHGEF2. Interacts (via protein kinase domain) with RFFL; involved in RIPK1 ubiquitination. Interacts with RNF34; involved in RIPK1 ubiquitination.

Alternative Nomenclature

Cell death protein RIP antibody

Receptor (TNFRSF) interacting serine threonine kinase 1 antibody

Receptor-interacting protein 1 antibody

Serine threonine protein kinase RIP antibody

Protein information supplied by UniProt
 


Accessories

Product Note Status Price
RIPK1 Antibody FITC RIPK1 Antibody FITC
RIPK1-FITC $350.00 *
RIPK1 Antibody BIOTIN RIPK1 Antibody BIOTIN
RIPK1-BIOTIN $350.00 *
RIPK1 Blocking Peptide RIPK1 Blocking Peptide
P-RIPK1 $145.00 *
RIPK1 Positive Control RIPK1 Positive Control
PC-RIPK1 $185.00 *
Display accessory details

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Browse these categories as well: Kinase / Phosphatases, Apoptosis, Apoptosis, Serine/Threonine Kinases, Receptor Interacting Proteins, Primaries