|
General Information
|
|
|
Product
|
Shadow Antibody
|
|
Description
|
Affinity Purified Shadow Antibody C-epitope
|
|
Verified Applications
|
CM, ELISA, ICC, IF, IHC, IP, WB
|
|
Host
|
Rabbit
|
|
Species Cross Reactivity
|
Drosophila melanogaster
|
|
Immunogen
|
Synthetic peptide corresponding to unique amino acid sequence near C-terminal of Shadow protein.
|
|
Accession #
|
NCBI: NP_650123
|
|
Physical Properties
|
|
|
Quantity
|
100 µg
|
|
Volume
|
200 µl
|
|
Concentration
|
0.7 µg/µl in antibody stabilization buffer
|
|
Form
|
Affinity Purified
|
|
Clonality
|
Polyclonal
|
|
Determinant
|
C-epitope
|
|
Storage
|
-20⁰C for long term storage
|
|
Application Protocol
|
|
Confocal Microscopy
|
1:200
|
|
DB
|
1:10,000
|
|
ELISA
|
1:10,000
|
|
Immunocytochemistry
|
1:200
|
|
Immunohistochemistry
|
1:200
|
|
Immunoprecipitation
|
1:200
|
|
Western Blot
|
1:500
|
|
Protein
|
|
Uniprot #
|
Q9VGH1
|
|
Overview
|
Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
|
|
Molecular Function
|
Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity
|
|
Subcellular Location
|
Mitochondrion membrane
|
|
Expression
|
Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.
|
|
Alternative Nomenclature
|
CG14728 antibody
Cyp315a1 antibody
CYPCCCXVA1 antibody
Cytochrome P450 315a1 mitochondrial antibody
Karmoisin ghost antibody
LP13573p antibody
sad antibody
Sad PA antibody
shade antibody
|
| |
|
Shadow Antibody FITC
Shadow Antibody BIOTIN
Shadow Blocking Peptide
Shadow Positive Control
