Shadow Antibody BIOTIN

General Information

Product

Shadow Antibody BIOTIN-Conjugated

Description

BIOTIN-Conjugated Shadow Antibody Mid-Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near mid region of Shadow protein.

Accession #

NCBI:  NP_650123

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

BIOTIN-Conjugated

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VGH1

Overview

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity

Subcellular Location

Mitochondrion membrane

Expression

Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and

Alternative Nomenclature

CG14728 antibody

Cyp315a1 antibody

CYPCCCXVA1 antibody

Cytochrome P450 315a1 mitochondrial antibody

Karmoisin ghost antibody

LP13573p antibody

sad antibody

Sad PA antibody

shade antibody