ULK3 Antibody

Product no.: ULK3-301AP

$285.00

Description

General Information
Product	ULK3 Antibody
Description	Affinity Purified Serine/Threonine-protein kinase ULK3 Antibody C-epitope
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide corresponding to a region within C terminal amino acids 452-472 of Mouse ULK3, coupled to a carrier protein.
Accession #	NCBI: NP_001258064.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	C-epitope
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	D3ZHP7
Overview	Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Molecular Function	ATP-binding; Nucleotide-binding; Serine/threonine-protein kinase
Subcellular Location	Cytoplasm
Expression	Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
Structure	Interacts (via protein kinase domain) with SUFU.
Alternative Nomenclature	DKFZP434C131 antibody FLJ90566 antibody Serine/threonine-protein kinase ULK3 antibody Ulk3 antibody unc 51 like kinase 3 (C. elegans) antibody Unc-51-like kinase 3 antibody

Protein information supplied by UniProt