VEGFR1 Antibody

General Information

Product

VEGFR1 Antibody

Description

Affinity Purified Vascular Endothelial Growth Factor Receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1250-1300 on human Vascular endothelial growth factor receptor 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P17948

Overview

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Molecular Function

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1 :Cell membrane; Single-pass type I membrane protein

Endosome Note: Autophosphorylation promotes ubiquitination and endocytosis.

Isoform 2 :Secreted 

Isoform 3 :Secreted

Isoform 4 :Secreted

Isoform 5 :Cytoplasm

Isoform 6 :Cytoplasm 

Isoform 7 :Cytoplasm 

Expression

Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).

Structure

The second and third Ig-like C2-type (immunoglobulin-like) domains are sufficient for VEGFA binding. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 7 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

Alternative Nomenclature

EC 2.7.10.1 

FLT 

FLT1 

Fms like tyrosine kinase 1 

FRT 

Soluble VEGF receptor 1 14 

Soluble VEGFR1 variant 2 

Soluble VEGFR1 variant 21 

Tyrosine protein kinase FRT 

Tyrosine protein kinase receptor FLT 

Vascular endothelial growth factor vascular permeability factor receptor 

Vascular permeability factor receptor 1 

VEGFR1