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General Information
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Product
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Protein Vestigial Antibody Affinity Purified
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Description
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Affinity Purified Vestigial Antibody
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Verified Applications
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ELISA, WB
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Host
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Rabbit
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Species Cross Reactivity
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Drosophila melanogaster
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Immunogen
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Synthetic peptide taken within amino acid region 200-250 on D. melanogaster Vestigial protein.
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Accession #
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NCBI: NP_523723.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.68 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Western Blot
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1:500
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Protein
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Uniprot #
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Q26366
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Overview
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Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.
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Molecular Function
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DNA binding; Protein C-terminus binding
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Subcellular Location
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Nucleus
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Expression
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Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.
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Alternative Nomenclature
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VG
VSTGL
VG21
DMEL/CG3830
CCG3830
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Protein Vestigial Antibody FITC
Protein Vestigial Antibody BIOTIN
Protein Vestigial Blocking Peptide
Protein Vestigial Positive Control
