General Information

Product

Sall4C Antibody

Description

Affinity Purified SALL4C Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human SALL4C protein.

Accession #

GenBank: AAO16567.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q6Y8G5

Overview

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.

Molecular Function

DNA binding; Metal ion binding; Transcription Regulation

Subcellular Location

Cytoplasm, Nucleus

Structure

Belongs to the sal C2H2-type zinc-finger protein family.

Contains 7 C2H2-type zinc fingers.

Alternative Nomenclature

AA407717 antibody

AL022809 antibody

AW536104 antibody

C330011P20Rik antibody

C78083 antibody

C78563 antibody

DRRS antibody

HSAL4 antibody

Sal-like Protein 4 antibody

Spalt like transcription factor 4 antibody

Tex20 antibody

Zinc finger protein 797 antibody

Zinc finger protein SALL4 antibody

ZNF797 antibody

General Information

Product

GSK3 beta Antibody

Description

Affinity Purified Glycogen Synthase Kinase-3 beta Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human GSK3 beta protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P49841

Overview

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.

Molecular Function

Developmental protein, Kinase, Serine/threonine-protein kinase, Signal tranduction inhibitor, Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.

Structure

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Glycogen Synthase Kinase 3 Beta antibody

GSK 3 beta antibody

Serine/threonine-protein kinase GSK3B antibody

General Information

Product

14-3-3 sigma Antibody

Description

Affinity Purified 14-3-3 protein sigma / STRATIFIN / SFN Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-220 on human 14-3-3 protein sigma / STRATIFIN / SFN protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P31947

Overview

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.1 Publication

p53-regulated inhibitor of G2/M progression.

Molecular Function

cadherin binding

identical protein binding

phosphoprotein binding

protein domain specific binding

protein kinase binding

protein kinase C inhibitor activity

Subcellular Location

Nucleus, secreted

Expression

Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Structure

Belongs to the 14-3-3 family.

Alternative Nomenclature

14 3 3 protein sigma antibody

1433S_HUMAN antibody

Epithelial cell marker protein 1 antibody

Er antibody

HME 1 antibody

Mkrn3 antibody

Mme1 antibody

RP23 137L22.11 antibody

SFN antibody

SFN protein antibody

Stratifin antibody

YWHAS antibody