General Information

Product

EHD1 Antibody

Description

Affinity Purified EH domain-containing protein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken wtihin amino acid region 515-565 on human EH-domain containing protein 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9H4M9

Overview

Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.

Molecular Function

Rab GTPase binding; ATP binding; calcium ion binding

Subcellular Location

Cell membrane, Endosome, Membrane

Expression

testis

Structure

Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization.

Alternative Nomenclature

CDABP0131 antibody

EH domain containing 1 antibody

FLJ42622 antibody

FLJ44618 antibody

H PAST antibody

PAST antibody

PAST1 antibody

Testilin antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 20-70 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Structure

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 80-130 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Expression

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody Mixed Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 50-100 and 80-130 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multiple Epitopes

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Expression

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody Mid-region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 50-100 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Expression

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

OGT1 Antibody

Description

Affinity Purified O-linked N-acetylglucosamine transferase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human O-linked N-acetylglucosamine transferase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15294

Overview

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity.

Molecular Function

Chromatin regulator, Glycosyltransferase, Transferase

Subcellular Location

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Expression

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

Structure

The TPR repeat domain is required for substrate binding and oligomerization.

Alternative Nomenclature

GlcNAc transferase

HRNT1 

O GlcNAc 

O GlcNAc transferase p110 subunit 

O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) 

UDP N acetylglucosamine peptide N

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit