General Information

Product

CD10 Antibody

Description

Affinity Purified Neprilysin Antibody Extreme N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Neprilysin protein.

Accession #

NCBI: NP_009218

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.78 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P08473

Overview

Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. angiotensin maturation; cellular response to UV; replicative senescence

Molecular Function

Hydrolase, Metalloprotease, Protease

Subcellular Location

Cell membrane, Membrane

Expression

Adipose tissue; duodenum; kidney; small intestine

Structure

Belongs to the peptidase M13 family

Alternative Nomenclature

Atriopeptidase antibody

CALLA antibody

CD10 antibody

Common acute lymphocytic leukemia antigen antibody

DKFZp686O16152 antibody

EC 3.4.24.11 antibody

Enkephalinase antibody

EPN antibody

gp100 antibody

Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody

Membrane metallo endopeptidase antibody

MGC126681 antibody

MGC126707 antibody

MME antibody

NEP antibody

Neprilysin antibody

Neutral endopeptidase antibody

SFE antibody

Skin fibroblast elastase antibody

General Information

Product

CD10 Antibody

Description

Affinity Purified Neprilysin Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Recombinant CD10 protein.

Accession #

NCBI: NP_009218

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P08473

Overview

Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. angiotensin maturation; cellular response to UV; replicative senescence.

Molecular Function

Hydrolase, Metalloprotease, Protease

Subcellular Location

Cell membrane, Membrane

Expression

Adipose tissue; duodenum; kidney; small intestine

Structure

Belongs to the peptidase M13 family

Alternative Nomenclature

Atriopeptidase antibody

CALLA antibody

CD10 antibody

Common acute lymphocytic leukemia antigen antibody

DKFZp686O16152 antibody

EC 3.4.24.11 antibody

Enkephalinase antibody

EPN antibody

gp100 antibody

Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody

Membrane metallo endopeptidase antibody

MGC126681 antibody

MGC126707 antibody

MME antibody

NEP antibody

Neprilysin antibody

Neutral endopeptidase antibody

SFE antibody

Skin fibroblast elastase antibody

General Information

Product

CD10 Antibody

Description

Affinity Purified Neprilysin Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Neprilysin protein.

Accession #

NCBI: NP_009218

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.78 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P08473

Overview

Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. angiotensin maturation; cellular response to UV; replicative senescence

Molecular Function

Hydrolase, Metalloprotease, Protease

Subcellular Location

Cell membrane, Membrane

Expression

Adipose tissue; duodenum; kidney; small intestine

Structure

Belongs to the peptidase M13 family

Alternative Nomenclature

Atriopeptidase antibody

CALLA antibody

CD10 antibody

Common acute lymphocytic leukemia antigen antibody

DKFZp686O16152 antibody

EC 3.4.24.11 antibody

Enkephalinase antibody

EPN antibody

gp100 antibody

Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody

Membrane metallo endopeptidase antibody

MGC126681 antibody

MGC126707 antibody

MME antibody

NEP antibody

Neprilysin antibody

Neutral endopeptidase antibody

SFE antibody

Skin fibroblast elastase antibody

General Information

Product

CD10 Antibody

Description

Affinity Purified Neprilysin Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Neprilysin protein.

Accession #

NCBI: NP_009218

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.78 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P08473

Overview

Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. angiotensin maturation; cellular response to UV; replicative senescence

Molecular Function

Hydrolase, Metalloprotease, Protease

Subcellular Location

Cell membrane, Membrane

Expression

Adipose tissue; duodenum; kidney; small intestine

Structure

Belongs to the peptidase M13 family

Alternative Nomenclature

Atriopeptidase antibody

CALLA antibody

CD10 antibody

Common acute lymphocytic leukemia antigen antibody

DKFZp686O16152 antibody

EC 3.4.24.11 antibody

Enkephalinase antibody

EPN antibody

gp100 antibody

Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody

Membrane metallo endopeptidase antibody

MGC126681 antibody

MGC126707 antibody

MME antibody

NEP antibody

Neprilysin antibody

Neutral endopeptidase antibody

SFE antibody

Skin fibroblast elastase antibody

General Information

Product

CD10 Antibody

Description

Affinity Purified Neprilysin Antibody Extreme C-epitope

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human Neprilysin protein.

Accession #

NCBI: NP_009218

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.78 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P08473

Overview

Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. angiotensin maturation; cellular response to UV; replicative senescence

Molecular Function

Hydrolase, Metalloprotease, Protease

Subcellular Location

Cell membrane, Membrane

Expression

Adipose tissue; duodenum; kidney; small intestine

Structure

Belongs to the peptidase M13 family

Alternative Nomenclature

Atriopeptidase antibody

CALLA antibody

CD10 antibody

Common acute lymphocytic leukemia antigen antibody

DKFZp686O16152 antibody

EC 3.4.24.11 antibody

Enkephalinase antibody

EPN antibody

gp100 antibody

Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody

Membrane metallo endopeptidase antibody

MGC126681 antibody

MGC126707 antibody

MME antibody

NEP antibody

Neprilysin antibody

Neutral endopeptidase antibody

SFE antibody

Skin fibroblast elastase antibody

General Information

Product

ENPP2 Antibody

Description

Affinity Purified ectonucleotide pyrophosphatase/phosphodiesterase 2 (Autotaxin) Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 778-818 on human Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) protein.

Accession #

GenBank: EAW91988.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

UPI0000EE3297

Overview

Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.

Molecular Function

alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity; lysophospholipase activity; nucleic acid binding; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; scavenger receptor activity; transcription factor binding; zinc ion binding

Subcellular Location

Secreted

Expression

Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Structure

Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Contains 2 SMB (somatomedin-B) domains.

Alternative Nomenclature

ATX antibody

ATX X antibody

Autotaxin antibody

Autotaxin t antibody

Ectonucleotide pyrophosphatase/phosphodiesterase 2 antibody

Enpp2 antibody

Extracellular lysophospholipase D antibody

FLJ26803 antibody

LysoPLD antibody

NPP2 antibody

PD IALPHA antibody

PDNP2 antibody

Phosphodiesterase I alpha antibody

Plasma lysophospholipase D antibody

General Information

Product

Cytokeratin 20 Antibody

Description

Affinity Purified keratin, type I cytoskeletal 20 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse

Immunogen

Synthetic peptide taken within amino acid region 300-350 human keratin, type I cytoskeletal 20 protein.

Accession #

NCBI:  NP_061883.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P35900

Overview

Plays a role in maintaining keratin filament organization in intestinal epithelia.  When phosphorlyated it is involved in the secretion of mucin in the small intestine.

Molecular Function

Structural constituent of cytoskeleton; Apoptosis

Subcellular Location

Cytoplasm

Expression

Mainly expressed in the itenstinal epithelium, expressed in luminal cells of colonic mucosa.  Also expressed in Merkel cells of keratinzed oral mucosa.

Structure

Heterotetramer of two type I and two type II keratins, associates with KRT8.

Alternative Nomenclature

CD20 antibody

CK20 antibody

Cytokeratin20 antibody

K20 antibody

Keratin 20 antibody

keratin 21, rat, homolog of antibody

Keratin antibody

Keratin type I cytoskeletal 20 antibody

KRT20 antibody

KRT21 antibody

Protein IT antibody

type I cytoskeletal 20 antibody

General Information

Product

Cytokeratin 20 Antibody

Description

Affinity Purified keratin, type I cytoskeletal 20 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse

Immunogen

Synthetic peptide taken within amino acid region 370-420 human keratin, type I cytoskeletal 20 protein.

Accession #

NCBI:  NP_061883.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P35900

Overview

Plays a role in maintaining keratin filament organization in intestinal epithelia.  When phosphorlyated it is involved in the secretion of mucin in the small intestine.

Molecular Function

Structural constituent of cytoskeleton; Apoptosis

Subcellular Location

Cytoplasm

Expression

Mainly expressed in the itenstinal epithelium, expressed in luminal cells of colonic mucosa.  Also expressed in Merkel cells of keratinzed oral mucosa.

Structure

Heterotetramer of two type I and two type II keratins, associates with KRT8.

Alternative Nomenclature

CD20 antibody

CK20 antibody

Cytokeratin20 antibody

K20 antibody

Keratin 20 antibody

keratin 21, rat, homolog of antibody

Keratin antibody

Keratin type I cytoskeletal 20 antibody

KRT20 antibody

KRT21 antibody

Protein IT antibody

type I cytoskeletal 20 antibody

General Information

Product

Calcitonin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 30-80 on human Calcitonin protein.

Accession #

GenBank: CAA26189.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01258

Overview

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Katacalcin is a potent plasma calcium-lowering peptide.

Molecular Function

Hormone; Calcitonin receptor binding; Identical protein binding

Subcellular Location

Secreted

Structure

Belongs to calcitonin family; Signal domain

Alternative Nomenclature

Alpha CGRP antibody

CALC1 antibody

CALCA antibody

Calcitonin related polypeptide alpha antibody

CGRP1 antibody

CT antibody

Katacalcin antibody

KC antibody

General Information

Product

Calcitonin Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 43-93 on human Calcitonin protein.

Accession #

GenBank: CAA26189.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01258

Overview

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Katacalcin is a potent plasma calcium-lowering peptide.

Molecular Function

Hormone; Calcitonin receptor binding; Identical protein binding

Subcellular Location

Secreted

Structure

Belongs to calcitonin family; Signal domain

Alternative Nomenclature

Alpha CGRP antibody

CALC1 antibody

CALCA antibody

calcitonin 1 antibody

calcitonin gene related peptide 1 antibody

Calcitonin related polypeptide alpha antibody

CGRP1 antibody

CT antibody

Katacalcin antibody

KC antibody