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General Information
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Product
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Aurora A Antibody
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Description
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Affinity Purified Aurora Kinase A Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 350-397 on human Aurora Kinase A protein.
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Accession #
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NCBI: NP_695208
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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| Immunohistochemistry |
1:50-1:200 |
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q3MHU2
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Overview
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Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes.
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Molecular Function
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ATP binding
histone serine kinase activity
protein heterodimerization activity
protein kinase activity
protein kinase binding
protein serine/threonine/tyrosine kinase activity
protein serine/threonine kinase activity
ubiquitin protein ligase binding
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Subcellular Location
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centrosome
spindle pole
cilium basal body
centriole
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Expression
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Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine.
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Structure
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Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
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Alternative Nomenclature
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AIK antibody
ARK1 antibody
AURA antibody
Aurora 2 antibody
Aurora A antibody
Aurora kinase A antibody
Aurora-related kinase 1 antibody
Aurora/IPL1 like kinase antibody
AURORA2 antibody
Breast tumor-amplified kinase antibody
BTAK antibody
hARK1 antibody
IAK antibody
IPL1 related kinase antibody
PPP1R47 antibody
Protein phosphatase 1, regulatory subunit 47 antibody
Serine/threonine kinase 15 antibody
Serine/threonine kinase 6 antibody
Serine/threonine-protein kinase aurora-A antibody
STK15 antibody
STK6 antibody
STK7 antibody
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Aurora A Antibody BIOTIN
Aurora A Antibody FITC
Aurora A Blocking Peptide
Aurora A Positive Control
