General Information
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Product
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Eprhin Receptor A4 Antibody Affinity Purified
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Description
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Affinity Purified Ephrin type-A receptor 4 Antibody
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Verified Applications
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ELISA, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse
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Immunogen
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Synthetic peptide taken within amino acid region 550-650 on human EphA R4 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.55-0.75 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Western Blot
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1:500
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Protein
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Uniprot #
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Q03137
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Overview
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Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
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Molecular Function
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ATP-binding, Nucleotide-binding
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Subcellular Location
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Cell membrane; Single-pass type I membrane protein
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Expression
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Highest expression in the adult brain and retina and also detectable in kidney, lung, skeletal muscle and thymus. Not detected in heart and liver.
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Structure
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Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts (via
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Alternative Nomenclature
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Cek 8 antibody
EK8 antibody
eph receptor a4 antibody
EPH-like kinase 8 antibody
EphA4 antibody
Ephrin receptor Eph A4 antibody
hEK8 antibody
Receptor protein tyrosine kinase HEK 8 antibody
Sek 1 antibody
SEK antibody
TYRO 1 protein tyrosine kinase antibody
Tyrosine-protein kinase receptor SEK antibody
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