Ephrin Receptor B1 Antibody

General Information

Product

Eprhin Receptor B1 Antibody

Description

Affinity Purified Ephrin type-B receptor 1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP ,WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 850-900 on mouse Eph BR1 protein.

Accession #

NCBI:  NP_775623

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

Q8CBF3

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in neural stem and progenitor cells in the dentate gyrus.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1. Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. Then ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.

Alternative Nomenclature

Cek 6 antibody

EK6 antibody

ELK antibody

Elkh antibody

Eph tyrosine kinase 2 antibody

EPH-like kinase 6 antibody

EPH2 antibody

EphB1 antibody

Ephrin receptor Eph B1 precursor antibody

Ephrin type B receptor 1 antibody

EPHT2 antibody

HEK 6 antibody

NET antibody

Neuronally expressed EPH related tyrosine kinase antibody

Tyrosine-protein kinase receptor EPH-2 antibody