FAK2 Antibody

Product no.: FAK2-201Y

$285.00

Description

General Information
Product	FAK2 Antibody
Description	Affinity Purified Protein-tyrosine kinase 2-beta / Focal adhesion Kinase 2 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Chicken
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 700-750 on human focal adhesion kinase 2 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.6 µg/µl in antibody stabilization buffer
Immunoglobulin	IgY
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q14289
Overview	Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Molecular Function	Kinase, Transferase, Tyrosine-protein Kinase
Subcellular Location	Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus
Expression	Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
Structure	Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain.
Alternative Nomenclature	CADTK antibody CAK beta antibody CAKB antibody Calcium dependent tyrosine kinase antibody Calcium regulated non receptor proline rich tyrosine kinase antibody Cell adhesion kinase beta antibody E430023O05Rik antibody EC 2.7.10.2 antibody FADK2 antibody FAK2 antibody Focal Adhesion Kinase 2 antibody MGC124628 antibody PKB antibody pp125FAK antibody Proline Rich Tyrosine Kinase 2 antibody Protein kinase B antibody Protein Tyrosine Kinase 2 Beta antibody PTK antibody PTK2B protein tyrosine kinase 2 beta antibody PYK 2 antibody RAFTK antibody RAFTK2 antibody Related adhesion focal tyrosine kinase antibody

Protein information supplied by UniProt