PDE5A Blocking Peptide

Product no.: P-PD5A

$155.00

General Information
Product	PDE5A Peptide
Description	Antigenic Blocking Peptide cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
Verified Applications	Immunodepletion, Immunocompetition
Peptide Description	Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.
Physical Properties
Quantity	250 µg
Volume	100 µl
Form	Antigenic Blocking Peptide
Storage	-20⁰C for long term storage
Application Protocol
Competition/Depletion assay	Refer to competition assay protocol
Protein
Uniprot #	O76074
Overview	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Molecular Function	Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding
Subcellular Location	cytosol
Expression	Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Alternative Nomenclature	5''-cyclic phosphodiesterase antibody CGB PDE antibody cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody cGMP binding/cGMP specific phosphodiesterase antibody cGMP specific 3'5' cyclic phosphodiesterase antibody cGMP specific phosphodiesterase PDE5A2 antibody cGMP specific phosphodiesterase type 5A antibody cGMP-binding cGMP-specific phosphodiesterase antibody cGMP-specific 3'' antibody CN5A antibody CN5N antibody PDE 5 antibody PDE 5A antibody PDE5A1 antibody Phosphodiesterase 5A antibody Phosphodiesterase 5A cGMP specific antibody Phosphodiesterase isozyme 5 antibody

Protein information supplied by UniProt

Fabgennix PDE5A Antibody has been used in 22 Publications
Rajagopal, Senthilkumar, et al. "Cytokine-Induced S-Nitrosylation of Soluble Guanylyl Cyclase and Expression of Phosphodiesterase 1A Contribute to Dysfunction of Longitudinal Smooth Muscle Relaxation." Journal of Pharmacology and Experimental Therapeutics 352.3 (2015): 509-518. PMID25704994	Immunofluorescence, Western Blot; Human
Kedia, George T., et al. "Expression and Distribution of Phosphodiesterase Isoenzymes in the Human Male Urethra." Urology (2015). PMID25704994	Immunohistochemistry, Western Blot; Human
Nordgaard, Julie Carøe, et al. "Role of Ser102 and Ser104 as Regulators of cGMP Hydrolysis by PDE5A." PloS one 9.9 (2014): e107627. PMID25247292	Immunofluorescence, Western Blot; Human
Akkerman, Sven et al. “PDE5 Inhibition Improves Object Memory in Standard Housed Rats but Not in Rats Housed in an Enriched Environment: Implications for Memory Models?” Ed. Patrizia Campolongo. PLoS ONE 9.11 (2014): e111692. PMC4221101	Western Blot; Rat
Witting, Nanna, et al. "Effect of sildenafil on skeletal and cardiac muscle in Becker muscular dystrophy." Annals of neurology 76.4 (2014): 550-557. PMID25042931	Western Blot; Mouse
Carøe Nordgaard, Julie et al. “Role of Ser102 and Ser104 as Regulators of cGMP Hydrolysis by PDE5A.” Ed. David D. Roberts. PLoS ONE 9.9 (2014): e107627. PMC4172485	Western Blot; Human
Mietens, Andrea, et al. "Phosphodiesterase 5 (PDE5) inhibition, ANP and NO rapidly reduce epididymal duct contractions, but long-term PDE5 inhibition in vivo does not." Molecular and cellular endocrinology 349.2 (2012): 145-153. PMID21996373	Immunohistochemistry, Western Blot; Huma, Rat
Shukla, Praveen, Chengwen Sun, and Stephen T. O’Rourke. “Melatonin Inhibits Nitric Oxide Signaling by Increasing PDE5 Phosphorylation in Coronary Arteries.” American Journal of Physiology - Heart and Circulatory Physiology 303.12 (2012): H1418–H1425. PMC3532536	Western Blot; Pig
Ückert, Stefan, et al. "Expression and distribution of phosphodiesterase isoenzymes in the Human seminal vesicles." The journal of sexual medicine 8.11 (2011): 3058-3065. PMID21810184	Immunohistochemistry, Western Blot; Human
Luong, Christina, et al. "Antenatal sildenafil treatment attenuates pulmonary hypertension in experimental congenital diaphragmatic hernia." Circulation 123.19 (2011): 2120-2131. PMID21537000	Western Blot; Rat
Adderley, Shaquria P. et al. “Identification of Cytosolic Phosphodiesterases in the Erythrocyte: A Possible Role for PDE5.” Medical Science Monitor : International Medical Journal of Experimental and Clinical Research 17.5 (2011): CR241–CR247. PMC3366467	Western Blot; Human, Rabbit
Ückert, S, et al. "Expression And Distribution Of Key Enzymes Of The Cyclic GMP Signaling In The Human Clitoris: Relation To Phosphodiesterase Type 5 (PDE5)." International Journal Of Impotence Research 23.5 (2011): 206-212. PMID21697861	Immunohistochemistry; Human
Mokni, Walid et al. “Concerted Regulation of cGMP and cAMP Phosphodiesterases in Early Cardiac Hypertrophy Induced by Angiotensin II.” Ed. Karl-Wilhelm Koch. PLoS ONE 5.12 (2010): e14227. PMC2997062	Immunohistochemistry, Western Blot; Rat
Murthy, K S. “Contractile Agonists Attenuate cGMP Levels by Stimulating Phosphorylation of cGMP-Specific PDE5; an Effect Mediated by RhoA/PKC-Dependent Inhibition of Protein Phosphatase 1.” British Journal of Pharmacology 153.6 (2008): 1214–1224. PMC2275441	Immunoprecipitation, Western Blot; Rabbit
Sasseville, Maxime, et al. "Partie 5 3’5’-cyclic guanosine monophosphate-specific phosphodiesterases in the porcine cumulus-oocyte complex." Endocrinology. 2008 Nov;149(11):5568-76. doi: 10.1210/en.2008-0547. Epub 2008 Jul 31 PMID18669600	Immunodepletion, Western Blot; Pig
Albrecht, Knut, et al. "Immunohistochemical distribution of cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the Human vagina: A potential forensic value?." Journal of forensic and legal medicine 14.5 (2007): 270-274. PMID17113813	Immunohistochemistry; Human
Ückert, Stefan, et al. "Immunohistochemical Description of Cyclic Nucleotide Phosphodiesterase (PDE) Isoenzymes in the Human Labia Minora." The Journal of Sexual Medicine 4.3 (2007): 602-608. PMID17498099	Immunofluorescence; Human
Werkström, Viktoria, et al. "Phosphodiesterase 5 In The Female Pig And Human Urethra: Morphological And Functional Aspects."BJU International 98.2 (2006): 414-423. PMID16626307	Immunohistochemistry; Human, Pig
Liu, Wu-Jiang, et al. "Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats." Asian journal of andrology 7.4 (2005): 381. PMID16281085	Western Blot; Rat
Wang, Lei, et al. "Activation of the PI3-K/Akt pathway mediates cGMP enhanced-neurogenesis in the adult progenitor cells derived from the subventricular zone." Journal of Cerebral Blood Flow & Metabolism 25.9 (2005): 1150-1158. PMID15815584	Immunohistochemistry, Western Blot; Rat
Ückert, Stefan, et al. "Cyclic adenosine monophosphate and cyclic guanosine monophosphate-phosphodiesterase isoenzymes in Human vagina: relation to nitric oxide synthase isoforms and vasoactive intestinal polypeptide-containing nerves." Urology 65.3 (2005): 604-610. PMID15780401	Immunohistochemistry; Human
Monfort, Pilar, María‐Dolores Muñoz, and Vicente Felipo. "Chronic hyperammonemia in vivo impairs long‐term potentiation in hippocampus by altering activation of cyclic GMP‐dependent‐protein kinase and of phosphodiesterase 5." Journal of neurochemistry 94.4 (2005): 934-942. PMID16092938	Western Blot; Rat

Publishing research using PD5A-101AP? Please let us know, we can cite the reference in this datasheet.

I am looking for an antibody for PDE5. I would like to use this antibody on rat lung tissue for immunohistochemistry staining, immunofluorescence staining and western blot. On the internet I can find some antibodies, but I’m not sure if they will work well enough on rat. Do you have an antibody which you can recommend for this?
1. Our PDE5A antibody (PD5A-101AP) will work in immunohistochemistry staining, immunofluorescence staining and western blot with rat tissues. If you visit the publications tab under the PD5A-101AP product page you can see a summary of several publications that used this antibody. PMID21996373, PMC2997062 and PMID15815584 are some papers that utilize both immunohistochemistry and Western Blot in rat samples. We also have a phospho specific PDE5A antibody (PPD5A-140AP) that has been validated in the same applications and tissues.