RASGRF2 Blocking Peptide

General Information

Product

RASGRF2 Peptide

Description

Antigenic Blocking Peptide Ras-specific guanine nucleotide-releasing factor 2 

Verified Applications

Immunodepletion, Immunocompetition

Peptide Description

Synthetic peptide taken within amino acid region 1100-1200 on human RASGRF2 protein.

Physical Properties

Quantity

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Storage

-20⁰C for long term storage

Application Protocol

Competition/Depletion assay

Refer to competition assay protocol

Protein

Uniprot #

O14827

Overview

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP.  Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation.

Molecular Function

Guanine-nucleotide releasing factor, Rho guayl-nucleotide exchange factor activity

Subcellular Location

Cytoplasm, Cell membrane; Peripheral membrane protein, Endoplasmic reticulum membrane; Peripheral membrane protein

Expression

Widely expressed with higher expression in the brain, followed by heart, lung, pancreas and kidney.  Detected in placenta.  Expressed in brain and lung.

Structure

Homooligomer and heterooligomer with RASGRF1.  Interacts with Ras and RAC1.  Interacts in a calcium-dependent manner with calmodulin.

Alternative Nomenclature

GRF2 antibody

Ras guanine nucleotide exchange factor 2 antibody

Ras-GRF2 antibody