TRF2 Blocking Peptide

General Information

Product

TRF2 Peptide

Description

Antigenic Blocking Peptide Telomeric Repeat-binding Factor 2 Mid-Region

Verified Applications

Immunocompeition, Immunodepletion

Peptide Description

Synthetic Peptide

Accession #

GenBank: AAB81135.1

Physical Properties

Quantity

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Competition/Depletion assay

Refer to competition assay protocol

Protein

Uniprot #

Q15554

Overview

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Molecular Function

Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding

Subcellular Location

Nucleus; Chromosome > telomere

Expression

Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.

Structure

Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct.

Alternative Nomenclature

Telomeric DNA binding protein antibody

Telomeric repeat binding factor 2 antibody

TERF 2 antibody

TRBF 2 antibody

TRF 2 antibody

TTAGGG repeat-binding factor 2 antibody