GBA Positive Control

Product no.: PC-GLCM

$200.00

Description

General Information
Product	GBA Positive Control
Description	Western Blot Positive Control Glucosylceramidase Antibody
Verified Applications	WB
Physical Properties
Quantity	5 Applications
Volume	Inquire
Concentration	The concentration of the sample is empirically set so that 20 µl per lane in Western and blotted with corresponding affinity purified antibody will give a clean band with a high signal to noise ratio on the gel.
Form	Western Blot Positive Control
Storage	-20⁰C for long term storage

Protein
Uniprot #	Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (PubMed:19279011). Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485).
Overview	glucosylceramidase activity glucosyltransferase activity hydrolase activity scavenger receptor binding signaling receptor binding steryl-beta-glucosidase activity
Molecular Function	Lysosome
Subcellular Location	Interacts with saposin-C (PubMed:10781797). Interacts with SCARB2 (PubMed:18022370). Interacts with TCP1 (PubMed:21098288). May interact with SNCA; may inhibit the glucosylceramidase activity (PubMed:23266198). Interacts with GRN; this interaction prevents aggregation of GBA-SCARB2 complex via interaction with HSPA1A upon stress (PubMed:27789271).
Expression	Belongs to the glycosyl hydrolase 30 family.
Structure	Lysosomal acid glucosylceramidase
Alternative Nomenclature	Lysosomal acid glucosylceramidase

Protein information supplied by UniProt.