PDE4A Antibody FITC

Product no.: PD4A-FITC

$425.00

Description
Publications

General Information
Product	PDE4A Antibody FITC-Conjugated
Description	FITC-Conjugated Pan Phosphodiesterase 4A Antibody
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Moose, Mouse, Rat
Immunogen	Synthetic cyclic peptide corresponding to the C-terminal region of PDE4A. Common to all PDE4A proteins.
Specificity	This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), PDE4A5 (109 kDa), PDE4A8 (106 kDa), AND PDE4Ax (10 kDa) and a 76 kDa testis specific A variant.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Form	FITC-Conjugated
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100-1:400
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:100-1:400
Immunofluorescence	1:100-1:400
Immunohistochemistry	1:100-1:400
Immunoprecipitation	1:250
Western Blot	1:1,000-1:2,500
Protein
Uniprot #	P27815
Overview	Hydrolyzes the second messenger cAMP which is a key regulator of many important physiological processes.
Molecular Function	cAMP
Subcellular Location	Isoform 1: perinuclear region, Isoform 2: ruffle membrane, Isoform 4: Peripheral membrane protein, Isoform 6: perinuclear region, Isoform 7: Membrane, Cytoplasm
Expression	Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressedi n the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
Structure	Belongs to cyclic nucleotide phosphodiesterase family. PDE4 subfamily
Alternative Nomenclature	5''-cyclic phosphodiesterase 4A antibody cAMP specific 3 5 cyclic phosphodiesterase 4A antibody DPDE2 antibody dunce like phosphodiesterase E2 antibody PDE 4A antibody PDE4 antibody PDE46 antibody PDE4A11 antibody Phosphodiesterase 4A antibody phosphodiesterase 4A cAMP specific antibody phosphodiesterase E2 dunce homolog antibody phosphodiesterase isozyme 4 antibody phosphodiesterase type 4A11 antibody

Protein information supplied by UniProt

Fabgennix PDE4A Antibody has been used in 44 Publications
Kedia, George T., et al. "Expression and Distribution of Phosphodiesterase Isoenzymes in the Human Male Urethra." Urology (2015). PMID25704994	Immunohistochemistry, Western Blot; Human
Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602	Immunohistochemistry; Human
Guo, Jiejie, et al. "Etazolate abrogates the lipopolysaccharide (LPS)-induced downregulation of the cAMP/pCREB/BDNF signaling, neuroinflammatory response and depressive-like behavior in mice." Neuroscience 263 (2014): 1-14. PMID24434771	Western Blot; Mouse
Zhai, Kui, et al. "Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms." Cellular signalling 26.5 (2014): 1001-1010. PMID23347325	Western Blot; Rat
Amanda, G. Vang, et al. "Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac." Biochemical Journal 456.3 (2013): 463-473. PMID24007532	Western Blot; Mouse
Wang, Zhen-Zhen et al. “RNA Interference-Mediated Phosphodiesterase 4D Splice Variants Knock-down in the Prefrontal Cortex Produces Antidepressant-Like and Cognition-Enhancing Effects.” British Journal of Pharmacology 168.4 (2013): 1001–1014. PMC3631387	Western Blot; Mouse
Yougbaré, Issaka, et al. "NCS 613 Exhibits Anti-Inflammatory Effects On Pbmcs From Lupus Patients By Inhibiting P38 MAPK And NF-Κb Signalling Pathways While Reducing Proinflammatory Cytokine Production." Canadian Journal Of Physiology & Pharmacology 91.5 (2013): 353-361. PMID23656347	Immunocytochemistry, Western Blot; Human
Schaal, Sandra Marie et al. “The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant Following Spinal Cord Injury.” Ed. Valentin Ceña. PLoS ONE 7.9 (2012): e43634. PMC. PMC3446989	Confocal Microscopy, Immunofluorescence, Immunohistochemistry, Western Blot; Rat
Haque, Mohammed Z., Paulo S. Caceres, and Pablo A. Ortiz. “β-Adrenergic Receptor Stimulation Increases Surface NKCC2 Expression in Rat Thick Ascending Limbs in a Process Inhibited by Phosphodiesterase 4.” American Journal of Physiology - Renal Physiology 303.9 (2012): F1307–F1314. PMC3518189	Western Blot; Rat
Yougbare, Issaka, et al. "NCS 613, a potent and specific PDE4 inhibitor, displays anti-inflammatory effects on Human lung tissues." American Journal of Physiology-Lung Cellular and Molecular Physiology 301.4 (2011): L441-L450 PMID21784969	Immunocytochemistry, Western Blot; Human