PDE4A8 Antibody

General Information

Product

PDE4A8 Antibody Affinity Purified

Description

Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 4A Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IMM, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide specific only for PDE4A8 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P27815

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Molecular Function

metal ion binding, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding

Subcellular Location

Isoform 1: Cytoplasm, perinuclear region; Isoform 2:  Cytoplasm, perinuclear region.  Cell projection, ruffle membrane; Isoform 4:  Peripheral membrane protein; Isoform 6:  Cytoplasm; perinuclear region; Isoform 7:  Cytoplasm, Membrane

Expression

Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Iso

Structure

Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.

Alternative Nomenclature

5''-cyclic phosphodiesterase 4A antibody

cAMP specific 3 5 cyclic phosphodiesterase 4A antibody

cAMP-specific 3'' antibody

DPDE2 antibody

dunce antibody

PDE46 antibody

PDE4A antibody

Phosphodiesterase 4 antibody

phosphodiesterase 4A cAMP specific antibody

phosphodiesterase E2 dunce homolog antibody