Phospho-PDE4A Antibody

Product no.: PPD4-140AP

$375.00

General Information
Product	Phospho-PDE4A Antibody
Description	Affinity Purified Phosphorylated Phosphodiesterase 4A (S140) Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rabbit, Rat
Immunogen	Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine residue.
Specificity	This antibody detects all known long-form variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with other members of the PDE family including PDE4B or PDE4D.
Notes	This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.57 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Apical cell membrane, Cytoplasm, Membrane
Expression	Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Structure	Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
Alternative Nomenclature	cAMP specific 3',5' cyclic phosphodiesterase 4D antibody DKFZp686M11213 antibody 5''-cyclic phosphodiesterase 4D antibody cAMP specific 3' 5' cyclic phosphodiesterase 4D antibody cAMP specific phosphodiesterase 4D antibody cAMP specific phosphodiesterase PDE4D6 antibody cAMP-specific 3'' antibody cyclic AMP phosphodiesterase antibody DPDE 3 antibody FLJ97311 antibody HSPDE4D antibody PDE 43 antibody Pde4d antibody PDE4DN2 antibody Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody Phosphodiesterase 4D cAMP specific Phosphodiesterase E3 dunce homolog Drosophila antibody STRK 1 antibody STRK1 antibody

Protein information supplied by UniProt

Fabgennix Phospho-PDE4A PKA Antibody has been used in 1 Publication

Zhu, Bing, et al. "Activation and induction of cyclic AMP phosphodiesterase (PDE4) in Rat pulmonary microvascular endothelial cells." Biochemical pharmacology 68.3 (2004): 479-491. PMID15242814

Western Blot; Human

Publishing research using PPD4-140AP? Please let us know, we can cite the reference in this datasheet.

We are interesting in your item, PPD4-140AP and I notice the immunogen is unclear to know the phosphorylation site of PDE4A. So could you please provide me the detailed information about the immunogen sequence and let me know which phosphorylation site of PDE4A does it recognize?
1. The phospho-PDE4A antibody was directed to Serine 140 in the rat/mouse PDE4A5 variant. This site is conserved in other PDE4A long form variants too.

Accessories

Product

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Status

Price

Phospho-PDE4A Antibody FITC

PPD4-FITC

$425.00 *

Phospho-PDE4A Antibody BIOTIN

PPD4-BIOTIN

$425.00 *

Phospho-PDE4A PKA site Blocking Peptide

P-PPD4

$195.00 *

Phospho-PDE4A PKA site Positive Control

PC-PPD4

$220.00 *

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