General Information

Product

PrLZ / TPD52 Antibody

Description

Affinity Purified Prostate leucine zipper isoform 5 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Prostate leucine zipper isoform 5 protein.

Accession #

NCBI:  NP_001020423.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P55327

Molecular Function

calcium ion binding, protein heterodimerization activity, protein homodimerization activity

Subcellular Location

cytoplasm, perinuclear region of cytoplasm, endoplasmic reticulum

Expression

Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary. 

Structure

Forms a homodimer or heterodimer with other members of the family. All isoforms interact with several 14-3-3 proteins.

Alternative Nomenclature

D52 antibody

Human 19.8 kDa protein mRNA, complete cds antibody

N8L antibody

PC 1 antibody

PC1/MRPS28 fusion protein antibody

PrLZ antibody

Prostate and colon associated protein antibody

Prostate leucine zipper antibody

Protein N8 antibody

TPD52 antibody

Tumor protein D52 antibody

1:150

General Information

Product

Sialoadhesin Antibody

Description

Affinity Purified CD169 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human Sialoadhesin protein.

Accession #

NCBI:  NP_075556

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9BZZ2

Overview

Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells.Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis.

Molecular Function

Carbohydrate binding; Cell adhesion; Endocytosis

Subcellular Location

Isoform 1: Cell membrane; Isoform 2: Secreted

Expression

Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophagrophages in rheumatoid arthritis.

Structure

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 16 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative Nomenclature

CD169 antibody

sialic acid binding Ig-like lectin 1, sialoadhesin antibody

Sialic acid-binding Ig-like lectin 1 antibody

Sialoadhesin antibody

Siglec 1 antibody

SN antibody