General Information

Product

Shibire Antibody

Description

Affinity Purified Shibire, isoform K Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (fruit fly)

Immunogen

Synthetic peptide taken within amino acid region 800-850 on D. melanogaster Shibire protein.

Accession #

NCBI:  NP_727910.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P27619

Overview

Microtubule-associated force-producing protein which is involved in the production of microtubule bundles and which is able to bind and hydrolyze GTP. Implicated in endocytic protein sorting.

Molecular Function

Hydrolase, Motor protein

Subcellular Location

Cytoplasm; Cytoskeleton

Structure

Contains 1 Dynamin-type G domain; Contains 1 GED domain; Contains 1 PH domain

Alternative Nomenclature

anon-WOO153538

CG18102

Ddyn

Ddyn3

Ddyn4

Shi

Shibere

General Information

Product

SPNS2 Antibody

Description

Affinity Purified Spingolipid transporter 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster, Monkey, Mouse, Rat

Immunogen

Synthetic amidated peptide from Human SPNS2 protein, the epitope location is extracytoplasmic between TMD9 and TMD10.

Human, Mouse: amino acids 482-499

D. melanogaster: amino acids 373-389

The peptide was synthesized at 90% HPLC purity, covalently modifed to achieve the desired antigenicity, and conjugated to carrier proteins.

Sequence:

ISD LIR QST KDS PLW EF

Accession #

GenBank:  AAH41772 

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8IVW8

Overview

Sphingolipid transporter required for migration of myocardial precursors.

Molecular Function

sphingolipid transporter activity

Subcellular Location

Membrane

Expression

esophagus,kidney,lung,gall bladder

Structure

Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.

Alternative Nomenclature

Protein spinster homolog 2 antibody

Spinster homolog 2 (Drosophila) antibody

SPNS 2 antibody

Spingolipid protein transporter 2 antibody

General Information

Product

Cytochrome P450 CYP307a1 Antibody Affinity Purified

Description

Affinity Purified Cytochrome P450 CYP307a1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 500-600 on D. melanogaster Spook protein.

Accession #

GenBank: AAQ05973.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9VRM7

Overview

Required for correct development of the embryonic midline glial cells which are necessary for the formation of distinct segmental commissures.

Molecular Function

heme binding, iron ion binding, monooxygenase activity, oxidoreductase activity

Subcellular Location

endoplasmic reticulum membrane, peripheral membrane protein

Alternative Nomenclature

Cytochrome P450 307a1 antibody

307a1 antibody

CG10594 antibody

CYP307A1-Dm antibody

CG10594 antibody

Dmspo antibody

Spo antibody

spo spook antibody

General Information

Product

Thickveins Antibody

Description

Affinity Purified Thickveins Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit Fly (Drosophila melanogaster)

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on C-terminus of pan Thickvein protein. Immunogenic peptide was covalently modified to achieve the desired antigenicity.

Sequence:

CVK GFR PPI PSR WQE DD

Accession #

NCBI:  NP_787992.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

predicted molecular weights: 57-64 kDa

Protein

Uniprot #

Q95SI0

Overview

ATP binding, protein kinase activity, receptor signaling protein serine/threonine kinase activity

Alternative Nomenclature

Thick veins protein antibody

tkv antibody

General Information

Product

Thickvein C Antibody

Description

Affinity Purified Thickveins, isoform C Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Thickvein C protein.

Accession #

NCBI:  NP_787990.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9VMT1

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Alternative Nomenclature

Thick veins protein antibody

tkv antibody

General Information

Product

trp Antibody Affinity Purified

Description

Affinity Purified Transient Receptor Potential isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Transient Receptor Potential isoform A protein.

Accession #

NCBI:  NP_476768.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:10,000

Protein

Uniprot #

P19334

Overview

A light-sensitive calcium channel that is required for inositide-mediated Ca2+ entry in the retina during phospholipase C (PLC)-mediated phototransduction. Ca2+ influx may then feed back and inhibit PLC, thereby facilitating phosphatidylinositol 4,5 bisphosphate (PIP2) recycling.

Molecular Function

Calcium channel, Ion channel, Calmodulin activity

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Expressed predominantly in the rhabdomeres of photoreceptor cells. Expressed in the third antennal segment and in the olfactory segment at approximately 70 hours after puparium formation during antennal development.

Structure

The C-terminus interacts with a PDZ domain of inaD to form the core of the inaD signaling complex. Other members of the complex include norpA (PLC), inaC (PKC), and possibly trpl, ninaC, FKBP59, calmodulin and rhodopsin. Forms homomultimers and heteromult

Alternative Nomenclature

DmTRP antibody

dTRP antibody

Transient receptor potential antibody

General Information

Product

Cadherin 74A Antibody

Description

Affinity Purified Cadherin 74A, isoform A Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 100-150 on D. melanogaster Cadherin 74A, isoform A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9VVG0

Molecular Function

Calcium ion binding; Protein homodimerization activity

Subcellular Location

Apical plasma membrane; Microvilus; Integral component of plasma membrane

Structure

Transmembrane helix

Alternative Nomenclature

Cadherin 74

Cadherin 74A

GD12413

GM24339

GG15818

GE22158

Cadherin-23-like

Cadherin 87A

General Information

Product

Phospho-SMOX Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 434-484 on D. melanogaster SMOX protein.

Accession #

GenBank: AAC83344.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q9TZQ2

Overview

axon guidance; dendrite morphogenesis;

determination of adult lifespan; imaginal disc-derived wing morphogenesis; mushroom body development; negative regulation of autophagy; neuron development; regulation of cell proliferation involved in imaginal disc-derived wing morphogenesis; regulation of mitotic cell cycle; transcription, DNA-templated; transforming growth factor beta receptor signaling pathway

Molecular Function

Chromatin DNA binding; Sequence-specific DNA binding transcription factor activity

Structure

Belongs to the dwarfin/SMAD family.

Contains 1 MH1 (MAD homology 1) domain.

Contains 1 MH2 (MAD homology 2) domain.

Alternative Nomenclature

C20orf16 antibody

Flavin containing amine oxidase antibody

Flavin containing spermine oxidase antibody

PAO 1 antibody

PAO antibody

PAOH1 antibody

Polyamine oxidase 1 antibody

Putative cyclin G1 interacting protein antibody

SMO antibody

Spermine oxidase antibody

General Information

Product

SMOX Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 434-484 on D. melanogaster SMOX protein.

Accession #

GenBank: AAC83344.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q9TZQ2

Overview

axon guidance;

dendrite morphogenesis;

determination of adult lifespan;

imaginal disc-derived wing morphogenesis ;

mushroom body development;

negative regulation of autophagy;

neuron development;

regulation of cell proliferation involved in imaginal disc-derived wing morphogenesis;

regulation of mitotic cell cycle;

transcription, DNA-templated;

transforming growth factor beta receptor signaling pathway;

Molecular Function

Chromatin DNA binding; Sequence-specific DNA binding transcription factor activity

Structure

Belongs to the dwarfin/SMAD family.

Contains 1 MH1 (MAD homology 1) domain.

Contains 1 MH2 (MAD homology 2) domain.

Alternative Nomenclature

C20orf16 antibody

Flavin containing amine oxidase antibody

Flavin containing spermine oxidase antibody

PAO 1 antibody

Polyamine oxidase 1 antibody

Putative cyclin G1 interacting protein antibody

SMO antibody

Spermine oxidase antibod

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 400-450 and 502-552 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1. Interacts with CITED2. Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody