General Information

Product

CTLA4 Antibody

Description

Affinity Purified Cytotoxic T-lymphocyte protein 4 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human CTLA4 protein.

Accession #

GenBank: AAL07473.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P16410

Overview

Inhibitory receptor acting as a major negative regulator of T-cell responses.  The affinity of CTLA4 for its natural B7 family lingands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory correceptor CD28.

Molecular Function

Adaptive Immunity

Subcellular Location

Cell membrane; Single pass type I membrane protein

Expression

Widely expressed with highest levels in lymphoid tissues.  Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory correceptor, on the cell surface following activation.

Structure

Homodimer; disfulfide-linked

Alternative Nomenclature

CD 152 antibody

CD antibody

CD152 antigen antibody

CD152 isoform antibody

Celiac disease 3 antibody

CELIAC3 antibody

CTLA-4 antibody

Cytotoxic T cell associated 4 antibody

Cytotoxic T lymphocyte associated serine esterase 4 antibody

GRD4 antibody

GSE antibody

ICOS antibody

IDDM12 antibody

Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody

General Information

Product

Themis Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-50 on Thymocyte-expressed molecule involved in selection protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N1K5

Overview

Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells.

Molecular Function

Developmental protein

Subcellular Location

Cytoplasm, nucleus

Alternative Nomenclature

C6orf190 antibody

C6orf207 antibody

MGC163388 antibody

Protein THEMIS antibody

SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody

SPOT antibody

THEMIS antibody

Thymocyte expressed molecule involved in selection antibody

Thymocyte selection pathway associated antibody

TSEPA antibody

General Information

Product

CD3 Gamma Antibody Affinity Purified

Description

Affinity Purified T-cell surface glycoprotein CD3 gamma chain Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 131-181 on human T-cell surface glycoprotein CD3 gamma protein.

Accession #

NCBI: NP_000064.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P09693

Overview

The CD3 complex mediates signal transduction.

Molecular Function

Protein heterodimerization activity; T cell receptor binding; Receptor signaling complex scaffold activity; Transmembrane signaling receptor activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

Alternative Nomenclature

CD3 gamma antibody

CD3g antibody

CD3g antigen gamma polypeptide (TiT3 complex) antibody

CD3g antigen gamma polypeptide antibody

CD3g molecule gamma (CD3 TCR complex) antibody

T-cell receptor T3 gamma chain antibody

T-cell surface glycoprotein CD3 gamma chain antibody

T3G antibody

General Information

Product

CD4 Antibody

Description

Affinity Purified T-cell surface glycoprotein CD4 isoform 1 precursor Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse

Immunogen

Synthetic peptide taken within amino acid region 270-320 on human CD4 protein.

Accession #

NCBI:  NP_000607.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01730

Overview

Accesory protein for MHC class-II antigen/T-cell receptor interaction.

Molecular Function

Adaptive Immunity; Host-virus interaction; Immunity

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Structure

Contains 3 Ig-like C2-type domains; Contains 1 Ig-like V-type domain

Alternative Nomenclature

CD 4 antibody

CD4 (L3T4) antibody

CD4 antigen (p55) antibody

CD4 antigen antibody

CD4 molecule antibody

CD4 receptor antibody

CD4+ Lymphocyte deficiency, included antibody

CD4mut antibody

L3T4 antibody

Leu3 antibody

Ly-4 antibody

Lymphocyte antigen CD4 antibody

p55 antibody

T cell differentiation antigen L3T4 antibody

T cell OKT4 deficiency, included antibody

T-cell surface antigen T4/Leu-3 antibody

T-cell surface glycoprotein CD4 antibody

W3/25 antigen antibody

General Information

Product

CD4 Antibody

Description

Affinity Purified T-cell surface glycoprotein CD4 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to Human CD4 aa 437-458 (C terminal). The selected peptide was post-synthetically modified to achieve highest antigenicity before couplingto carrier protein using hetero bifunctional cross linker for Immunogen preparation.

Sequence:

RLLSEKKTCQCPHRFQKTCSPI

Accession #

NCBI:  NP_000607.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500. Predicted Molecular weight: 51 kDa

CD4-112AP labels a band of 28 kDa, the identity of this band is not fully understood, but may represent a proteolytic degradation product of CD4.

Protein

Uniprot #

P01730

Overview

Accesory protein for MHC class-II antigen/T-cell receptor interaction.

Molecular Function

Adaptive Immunity; Host-virus interaction; Immunity

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Structure

Contains 3 Ig-like C2-type domains; Contains 1 Ig-like V-type domain

Alternative Nomenclature

CD 4 antibody

CD4 (L3T4) antibody

CD4 antigen (p55) antibody

CD4 antigen antibody

CD4 molecule antibody

CD4 receptor antibody

CD4+ Lymphocyte deficiency, included antibody

CD4mut antibody

L3T4 antibody

Leu3 antibody

Ly-4 antibody

Lymphocyte antigen CD4 antibody

p55 antibody

T cell differentiation antigen L3T4 antibody

T cell OKT4 deficiency, included antibody

T-cell surface antigen T4/Leu-3 antibody

T-cell surface glycoprotein CD4 antibody

W3/25 antigen antibody

General Information

Product

CD99 Antibody

Description

Affinity Purified CD99 Antigen Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Baboon, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 50-100 to human CD99 antigen.

Accession #

GenBank: EAW98700.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI0000EE42C0

Overview

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Molecular Function

Cell Adhesion

Subcellular Location

Membrane

Structure

Belongs to CD99 family

Alternative Nomenclature

CD99 antibody

CD99 antigen antibody

CD99 molecule antibody

CD99X antibody

E2 antigen antibody

Ewing Sarcoma antibody

MIC 2X antibody

MIC 2Y antibody

MIC2 antibody

MSK5X antibody

Protein MIC2 antibody

Surface antigen MIC2 antibody

T cell surface glycoprotein E2 antibody

General Information

Product

CD99 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 97-147 to human CD99 antigen.

Accession #

GenBank: EAW98700.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI0000EE42C0

Overview

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Molecular Function

Cell Adhesion

Subcellular Location

Membrane

Structure

Belongs to CD99 family

Alternative Nomenclature

12E7 antibody

CD99 antibody

CD99 antigen antibody

CD99 molecule antibody

CD99X antibody

E2 antigen antibody

Ewing Sarcoma antibody

MIC 2X antibody

MIC 2Y antibody

MIC2 antibody

MSK5X antibody

Protein MIC2 antibody

Surface antigen MIC2 antibody

T cell surface glycoprotein E2 antibody

General Information

Product

CD2 Antibody

Description

Affinity Purified T-cell surface antigen CD2 Antibody C-epitope

Verified Applications

ELISA, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Predicted: Mouse, Rat

Immunogen

Accession #

NCBI:  NP_001758

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P06729

Overview

Interacts with lymphocyte function associated antigen and CD48/BCM1 to mediate adhesion between T-cells and other cells.

Molecular Function

Cell adhesion

Subcellular Location

Membrane; Single-pass type I membrane protein.

Structure

Cytoplasmic domain is implicated in signaling function.  Contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain

Alternative Nomenclature

CD 2 antibody

CD2 antigen (p50), sheep red blood cell receptor antibody

CD2 antigen antibody

CD2 molecule antibody

Erythrocyte receptor antibody

FLJ46032 antibody

LFA2 antibody

LFA3 receptor antibody

Ly-37 antibody

Lymphocyte function antigen 2 antibody

OTTHUMP00000024366 antibody

p50 antibody

Rosette receptor antibody

Sheep erythrocyte receptor antibody

Sheep red blood cell receptor antibody

SRBC antibody

T cell surface antigen CD2 antibody

T cell surface antigen T11/Leu 5 antibody

T11 antibody

General Information

Product

CD2 Antibody

Description

Affinity Purified T-cell surface antigen CD2 precursor Antibody Extreme C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Predicted: Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acid region 3335-351 on human T-cell surface antigen CD2 protein.

Accession #

NCBI:  NP_001758

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P06729

Overview

Interacts with lymphocyte function associated antigen and CD48/BCM1 to mediate adhesion between T-cells and other cells.

Molecular Function

Cell adhesion

Subcellular Location

Membrane; Single-pass type I membrane protein.

Structure

Cytoplasmic domain is implicated in signaling function.  Contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain

Alternative Nomenclature

CD 2 antibody

CD2 antigen (p50), sheep red blood cell receptor antibody

Erythrocyte receptor antibody

FLJ46032 antibody

LFA-2 antibody

LFA-3 receptor antibody

Ly-37 antibody

Lymphocyte function antigen 2 antibody

p50 antibody

Rosette receptor antibody

Sheep erythrocyte receptor antibody

T cell surface antigen CD2 antibody

T cell surface antigen T11/Leu 5 antibody

General Information

Product

CD3 epsilon Antibody

Description

Affinity Purified T-cell surface glycoprotein CD3 epsilon chain precursor Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequence on Human CD3 epsilon protein.

Accession #

NCBI:  NP_000724.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P07766

Overview

Mediates signal transduction

Molecular Function

Receptor; GPCR signaling pathway

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer coexprassed at the cell surface with invariant subunits of CD3 labeled gamma, delta epsilon, zeta, eta.

Alternative Nomenclature

CD3 TCR complex antibody

IMD18 antibody

T cell antigen receptor complex epsilon subunit of T3 antibody

T-cell surface antigen T3/Leu-4 epsilon chain antibody

T3E antibody

TCRE antibody

TiT3 complex antibody