General Information

Product

CD3 epsilon Antibody

Description

Affinity Purified T-cell surface glycoprotein CD3 epsilon chain Antibody Mid-region

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Acomys cahirinus, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human CD3 epsilon protein.

Accession #

NCBI:  NP_000724.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P07766

Overview

Mediates signal transduction

Molecular Function

Receptor; GPCR signaling pathway

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer coexprassed at the cell surface with invariant subunits of CD3 labeled gamma, delta epsilon, zeta, eta.

Alternative Nomenclature

CD3 TCR complex antibody

IMD18 antibody

T cell antigen receptor complex epsilon subunit of T3 antibody

T-cell surface antigen T3/Leu-4 epsilon chain antibody

T3E antibody

TCRE antibody

TiT3 complex antibody

General Information

Product

CD3 epsilon Antibody Affinity Purified

Description

Affinity Purified T-cell surface glycoprotein CD3 epsilon chain precursor Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

C-epitope synthetic peptide corresponding to unique amino acid sequence on CD3 epsilon protein.

Accession #

NCBI:  NP_000724.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P07766

Overview

Mediates signal transduction

Molecular Function

Receptor; GPCR signaling pathway

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer coexprassed at the cell surface with invariant subunits of CD3 labeled gamma, delta epsilon, zeta, eta.

Alternative Nomenclature

CD3 TCR complex antibody

IMD18 antibody

T cell antigen receptor complex epsilon subunit of T3 antibody

T-cell surface antigen T3/Leu-4 epsilon chain antibody

T3E antibody

TCRE antibody

TiT3 complex antibody

General Information

Product

CD3 epsilon Antibody

Description

Affinity Purified T-cell surface glycoprotein CD3 epsilon chain precursor Antibody Extreme C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Extreme C-epitope synthetic peptide corresponding to unique amino acid sequence on CD3 epsilon protein.

Accession #

NCBI:  NP_000724.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P07766

Overview

Mediates signal transduction

Molecular Function

Receptor; GPCR signaling pathway

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer coexprassed at the cell surface with invariant subunits of CD3 labeled gamma, delta epsilon, zeta, eta.

Alternative Nomenclature

CD3 TCR complex antibody

IMD18 antibody

T cell antigen receptor complex epsilon subunit of T3 antibody

T-cell surface antigen T3/Leu-4 epsilon chain antibody

T3E antibody

TCRE antibody

TiT3 complex antibody

General Information

Product

Lck Antibody

Description

Affinity Purified Lymphocyte cell-specific protein-tyrosine kinase Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-539 on human Lymphocyte cell-specific protein-tyrosine kinase.

Accession #

GenBank: AAH13200.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06239

Overview

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex.

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Membrane

Expression

Expressed specifically in lymphoid cells.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

Alternative Nomenclature

IMD22 antibody

LCK antibody

Lck p56 antibody

LCK proto-oncogene, Src family tyrosine kinase antibody

Leukocyte C-terminal Src kinase antibody

LSK antibody

Lymphocyte cell specific protein tyrosine kinase antibody

Membrane associated protein tyrosine kinase antibody

Oncogene lck antibody

p56(LSTRA) protein tyrosine kinase antibody

p56-LCK antibody

pp58 lck antibody

Protein YT16 antibody

Protooncogene tyrosine protein kinase LCK antibody

T lymphocyte specific protein tyrosine kinase p56lck antibody

Tyrosine-protein kinase Lck antibody

YT 16 antibody

General Information

Product

CD39 Antibody

Description

Affinity Purified EctoNucleoside TriPhosphate Diphosphohydrolase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 440-490 on human EctoNucleoside TriPhosphate Diphosphohydrolase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P49961

Overview

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP.

Molecular Function

blood coagulation

cell adhesion

nucleobase-containing small molecule catabolic process

Subcellular Location

Membrane; Multi-pass membrane protein

Expression

Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.

Structure

Belongs to the GDA1/CD39 NTPase family.

Alternative Nomenclature

ATPDase antibody

CD 39 antibody

CD39 antigen antibody

Ecto apyrase antibody

Ecto ATP diphosphohydrolase antibody

Ecto-ATPase 1 antibody

Ecto-ATPDase 1 antibody

Ectonucleoside triphosphate diphosphohydrolase 1 antibody

ENTPD1 antibody

Lymphoid cell activation antigen antibody

NTPDase 1 antibody

SPG64 antibody

General Information

Product

CD39 Antibody

Description

Affinity Purified EctoNucleoside TriPhosphate Diphosphohydrolase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide taken within amino acid region 380-430 on mouse EctoNucleoside TriPhosphate Diphosphohydrolase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P55772

Overview

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

Molecular Function

8-oxo-dGTP phosphohydrolase activity

adenosine-diphosphatase activity

ATPase activity

ATP binding

dATP phosphohydrolase activity

dCTP phosphohydrolase activity

dGTP phosphohydrolase activity

dTTP phosphohydrolase activity

dUTP phosphohydrolase activity

Subcellular Location

Membrane

Expression

Homodimer; disulfide-linked.

Structure

Belongs to the GDA1/CD39 NTPase family.

Alternative Nomenclature

ATPDase antibody

CD 39 antibody

CD39 antigen antibody

Ecto apyrase antibody

Ecto ATP diphosphohydrolase antibody

Ecto-ATPase 1 antibody

Ecto-ATPDase 1 antibody

Ectonucleoside triphosphate diphosphohydrolase 1 antibody

ENTPD 1 antibody

Lymphoid cell activation antigen antibody

NTPDase 1 antibody

SPG64 antibody

General Information

Product

CD45 Antibody

Description

Affinity Purified Receptor-type tyrosine-protein phosphatase C Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1100-1150 on human Receptor-type tyrosine-protein phosphatase C.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P08575

Subcellular Location

Cell membrane, Membrane

Structure

Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

B220 antibody
CD45 antigen antibody
CD45R antibody
GP180 antibody
LCA antibody
Leukocyte common antigen antibody
loc antibody
Ly-5 antibody
LY5 antibody
Lyt-4 antibody
Protein tyrosine phosphatase receptor type c polypeptide antibody
Ptprc antibody
Receptor-type tyrosine-protein phosphatase C antibody
T200 antibody
T200 glycoprotein antibody
T200 leukocyte common antigen antibody

General Information

Product

CD8 alpha Antibody

Description

Affinity Purified T-cell surface glycoprotein CD8 Alpha chain Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human T-cell surface glycoprotein CD8 Alpha chain.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P01732

Molecular Function

coreceptor activity

MHC class I protein binding

MHC class I protein complex binding

protein kinase binding

Subcellular Location

Cell membrane, Membrane, Secreted

Structure

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

alpha polypeptide (p32) antibody
CD8 antibody
CD8 antigen alpha polypeptide antibody
CD8 antigen alpha polypeptide (p32) antibody
CD8A antigen antibody
CD8A molecule antibody
CD8A_HUMAN antibody
Leu2 antibody
Leu2 T lymphocyte antigen antibody
Ly3 antibody
LYT3 antibody
MAL antibody
OKT8 T cell antigen antibody
p32 antibody
T cell antigen Leu2 antibody
T cell co receptor antibody
T-cell surface glycoprotein CD8 alpha chain antibody
T-lymphocyte differentiation antigen T8/Leu-2 antibody
T8 T cell antigen antibody

General Information

Product

CD8 Antibody

Description

Affinity Purified T-cell surface glycoprotein CD8 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 70-120 on human T-cell surface glycoprotein CD8 Beta chain.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P10966

Molecular Function

coreceptor activity

MHC class I protein binding

Subcellular Location

Cell membrane, Membrane, Secreted

Structure

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

CD8b antigen antibody
CD8 antigen beta polypeptide 1 (p37) antibody
CD8 beta antibody
CD8b antibody
CD8b molecule antibody
CD8B1 antibody
Leu2 antibody
Ly3 antibody
LYT3 antibody
P37 antibody
T cell surface glycoprotein CD8 beta chain antibody
T lymphocyte surface glycoprotein beta chain antibody