General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 502-552 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 131-181 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1. Interacts with CITED2. Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

C-Myc Antibody

Description

Affinity Purified myc proto-oncogene protein Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human cMyc protein.

Accession #

NCBI:  NP_002458

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01106

Overview

Transcription factor that binds DNA in non-specific manner.  Also specifically recognizes the core sequence 5'CAC[GA]TG-3'.  Activates transcription of growth-related genes.

Molecular Function

Activator; Transcription regulation; DNA-binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Myc proto oncogene protein antibody

myc-related translation/localization regulatory factor antibody

MYCC antibody

Myelocytomatosis oncogene antibody

Niard antibody

Nird antibody

Oncogene Myc antibody

Proto-oncogene c-Myc antibody

Protooncogene homologous to myelocytomatosis virus antibody

RNCMYC antibody

Transcription factor p64 antibody

Transcriptional regulator Myc-A antibody

V-Myc avian myelocytomatosis viral oncogene homolog antibody

General Information

Product

C-Myc Antibody Affinity Purified

Description

Affinity Purified myc proto-oncogene protein Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 400-454 on human cMyc protein.

Accession #

NCBI:  NP_002458

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01106

Overview

Transcription factor that binds DNA in non-specific manner.  Also specifically recognizes the core sequence 5'CAC[GA]TG-3'.  Activates transcription of growth-related genes.

Molecular Function

Activator; Transcription regulation; DNA-binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Myc proto oncogene protein antibody

myc-related translation/localization regulatory factor antibody

MYCC antibody

Myelocytomatosis oncogene antibody

Niard antibody

Nird antibody

Oncogene Myc antibody

Proto-oncogene c-Myc antibody

Protooncogene homologous to myelocytomatosis virus antibody

RNCMYC antibody

Transcription factor p64 antibody

Transcriptional regulator Myc-A antibody

V-Myc avian myelocytomatosis viral oncogene homolog antibody

General Information

Product

Punt Antibody

Description

Affinity Purified Punt receptor Serine/Threonine kinase (punt) Antibody

Verified Applications

ELISA, ICC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to C terminal amino acids 497-515 of Drosophila melanogaster PUNT protein. Peptides were amidated for conjugation.

Sequence:

QLLIKNHTNIDDAKESTNC

Accession #

GenBank: L38495.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q24468

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Activin receptor complex, Plasma membrane

Structure

Contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 88CD antibody
Activin receptor type II antibody
Atr antibody
Atr88CD antibody
AtrII antibody
dlhC antibody
Dorsal holes C antibody
j5A5 antibody
Pun antibody
Put antibody
STKC antibody
TGF beta l antibody
TGF beta type receptor like antibody
Tgf r antibody

General Information

Product

Punt Antibody

Description

Affinity Purified Punt receptor Serine/Threonine kinase (punt) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Punt protein.

Accession #

GenBank: L38495.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q24468

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Activin receptor complex, Plasma membrane

Structure

Contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 88CD antibody
Activin receptor type II antibody
Atr antibody
Atr88CD antibody
AtrII antibody
dlhC antibody
Dorsal holes C antibody
j5A5 antibody
Pun antibody
Put antibody
STKC antibody
TGF beta l antibody
TGF beta type receptor like antibody
Tgf r antibody

General Information

Product

MAPK1 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P28482

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis.

Molecular Function

Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm › cytoskeleton › spindle

Nucleus

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Cytoplasm

Structure

Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4,

Alternative Nomenclature

ERK antibody
ERK-2 antibody
ERT1 antibody
Extracellular signal-regulated kinase 2 antibody
MAP kinase 1 antibody

MAP kinase isoform p42 antibody
MAPK 1 antibody

Mapk1 antibody
MAPK2 antibody
Mitogen-activated protein kinase 1 antibody
P38 antibody
P40 antibody
P41 antibody
p42-MAPK antibody
P42MAPK antibody
PRKM1 antibody
protein kinase, mitogen-activated, 1 antibody

General Information

Product

MAPK3 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P27361

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

Mitogen-activated protein kinase 3 Antibody MAP kinase 3 Antibody

MAPK 3 Antibody

ERT2 Antibody

Extracellular signal-regulated kinase 1 Antibody ERK-1 Antibody

Insulin-stimulated MAP2 kinase Antibody

MAP kinase isoform p44 Antibody p44-MAPK Antibody Microtubule-associated protein 2 kinase Antibody

p44-ERK1 Antibody

MAPK3 Antibody

ERK1 Antibody

PRKM3 Antibody

General Information

Product

MAPK6 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 480-530 on human Mitogen-activated protein kinase 6.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q16659

Overview

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Expression

Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.

Structure

In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

ERK3 antibody
Extracellular signal regulated kinase p97 antibody
Extracellular signal-regulated kinase 3 antibody
HsT17250 antibody
MAP kinase 6 antibody
MAP kinase isoform p97 antibody
Mitogen activated protein kinase 6 antibody
p97 MAPK antibody
PRKM6 antibody
Protein kinase mitogen activated 6 antibody