General Information

Product

Pan PDE5A Antibody

Description

cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human

Immunogen

Recombinant protein common to all PDE5A variants corresponding to human PDE5A protein.

Accession #

NCBI:  NP_001074

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500-1:1,000

Protein

Uniprot #

O76074

Overview

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

cytosol

Expression

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Alternative Nomenclature

5''-cyclic phosphodiesterase antibody

CGB PDE antibody

cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody

cGMP binding/cGMP specific phosphodiesterase antibody

cGMP specific phosphodiesterase PDE5A antibody

cGMP specific phosphodiesterase type 5A antibody

cGMP-specific 3'' antibody

CN5A antibody

CN5N antibody

PDE 5 antibody

PDE 5A antibody

PDE5A antibody

Phosphodiesterase 5A antibody

Phosphodiesterase 5A cGMP specific antibody

Phosphodiesterase isozyme 5 antibody

General Information

Product

MAPK9 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 9 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 370-420 on human Mitogen-activated protein kinase 9.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P45984

Overview

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Nucleus

Structure

Interacts with MECOM and DCLK2. Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with NFATC4. Interacts with ATF7; the interaction does not phosphorylate ATF7 but acts as a docking site for ATF7-associated partners such as JUN. Interacts with BCL10. Interacts with CTNNB1 and GSK3B.

Alternative Nomenclature

c Jun kinase 2 antibody

JNK 55 antibody

JNK2 alpha antibody

JNK2 antibody

JNK2 beta antibody

Jun kinase antibody

MAP kinase 9 antibody

P54a antibody

p54aSAPK antibody

PRKM9 antibody

SAPK alpha antibody

Stress activated protein kinase 1a antibody

Stress-activated protein kinase JNK2 antibody

General Information

Product

GAPDH Antibody

Description

Affinity Purified Glyceraldehyde-3-Phosphate dehydrogenase isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on mouse Glyceraldehyde-3-phosphate dehydrogenase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P16858

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain;cerebullum;heart;kidney;liver;skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc-1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol 3 phosphate dehydrogenase cytosolic antibody

Glycerol 3 phosphate dehydrogenase soluble antibody

Glycerol-3-phosphate dehydrogenase [NAD+] antibody

Glycerphosphate dehydrogenase antibody

GPD-C antibody

gpd1 antibody

Gpd3 antibody

GPDA_HUMAN antibody

Gpdc antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

KIAA4010 antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody

General Information

Product

GAPDH Antibody

Description

Affinity Purified Glyceraldehyde-3-Phosphate dehydrogenase isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human Glyceraldehyde-3-phosphate dehydrogenase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P04406

Overview

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively

Molecular Function

Oxidoreductase, Transferase

Subcellular Location

Cytoplasm, Cytoskeleton, Nucleus

Expression

brain;cerebullum;heart;kidney;liver;skeletal muscle

Structure

Homotetramer

Alternative Nomenclature

AI747587 antibody

cytoplasmic antibody

EC 1.1.1.8 antibody

FLJ26652 antibody

G3PD antibody

Gdc-1 antibody

Gdp1 antibody

Glycerol 3 phosphate dehydrogenase 1 antibody

Glycerol 3 phosphate dehydrogenase cytosolic antibody

Glycerol 3 phosphate dehydrogenase soluble antibody

Glycerol-3-phosphate dehydrogenase [NAD+] antibody

Glycerphosphate dehydrogenase antibody

GPD-C antibody

gpd1 antibody

Gpd3 antibody

GPDA_HUMAN antibody

Gpdc antibody

GPDH antibody

GPDH-C antibody

HTGTI antibody

KIAA4010 antibody

MGC93453 antibody

MGPD antibody

mKIAA4010 antibody