General Information

Product

LAMP1 Antibody

Description

Affinity Purified Lysosome-associated membrane glycoprotein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human LAMP1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11279

Overview

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

Molecular Function

Enzyme binding

Subcellular Location

Cell membrane, single-pass type I membrane protein, endosome membrane, lysosome membrane (Note:This protein shuttles between lysosomes, endosomes, and plasma membrane. Colocalizes with OSBPL1A at the late endosome)

Alternative Nomenclature

CD107 antigen like family member A antibody

LAMP 1 antibody

LAMPA antibody

LGP120 antibody

lgpA antibody

Lysosomal Associated Membrane Protein 1 antibody

General Information

Product

Lysozyme Antibody

Description

Affinity Purified Homo sapiens lysozyme gene Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Partially purified full-length lysozyme protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P61626

Overview

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

Molecular Function

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Subcellular Location

Secreted

Structure

Monomer

Alternative Nomenclature

4 beta N acetylmuarmidase C antibody

1 antibody

4-beta-N-acetylmuramidase C antibody

EC 3.2.1.17 antibody

LYSC_HUMAN antibody

Lysosyme antibody

Lysozyme (renal amyloidosis) antibody

Lysozyme C antibody

LYZ antibody

LZM antibody

Renal amyloidosis antibody

General Information

Product

mTOR Antibody Affinity Purified

Description

Affinity Purified Serine/threonine-protein kinase mTOR Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-480 on mTOR protein.

Accession #

NCBI:  NP_063971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P42346

Overview

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Alternative Nomenclature

FK506 binding protein 12 rapamycin associated protein 1 antibody

FKBP rapamycin associated protein antibody

FRAP1 antibody

FRAP2 antibody

mTOR antibody

RAFT1 antibody

Rapamycin target protein antibody

RAPT1 antibody

Serine/threonine-protein kinase mTOR antibody

General Information

Product

LAMP2 Antibody

Description

Affinity Purified Lysosome-associated membrane glycoprotein 2 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 260-310 on human lysosomal membrane glycoprotein-2.

Accession #

GenBank: AAB41647.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P13473

Overview

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion

Molecular Function

enzyme binding

Subcellular Location

Cell membrane, Endosome, Lysosome, Membrane

Expression

LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle.LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.

Structure

Belongs to the LAMP family

Alternative Nomenclature

CD107 antigen-like family member B antibody

CD107b antibody

LAMP 2 antibody

LAMP 2C antibody

LAMPB antibody

LGP110 antibody

Lysosome associated membrane protein 2 antibody

MAC3 antibody

General Information

Product

Bcl-XL Antibody

Description

Affinity Purified Bcl-2-like protein 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human BCL-2-like protein 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q07817

Overview

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F1F0 activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis.

Molecular Function

BH3 domain binding; Identical protein binding; Protein heterodimerization and homodimerization activity; protein kinase binding

Subcellular Location

Mitochondrion inner membrane

Mitochondrion outer membrane

Mitochondrion matrix

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane

Cytoplasm › cytosol

Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

Nucleus membrane ; Single-pass membrane protein ; Cytoplasmic side

Note: After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner. Localizes to the centrosome when phosphorylated at Ser-49.

Expression

Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Structure

The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.

Alternative Nomenclature

Apoptosis regulator Bcl X antibody

B cell lymphoma 2 like antibody

B2CL1_HUMAN antibody

Bcl 2 like 1 protein antibody

Bcl X antibody

Bcl xL antibody

BCL XL/S antibody

Bcl xS antibody

Bcl-2-like protein 1 antibody

Bcl2 Like 1 antibody

Bcl2 related gene antibody

BCL2L antibody

BCLX antibody

PPP1R52 antibody

Protein phosphatase 1 regulatory subunit 52 antibody

General Information

Product

SQSTM1 / p62 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-440 on human Sequestosome-1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13501

Overview

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Molecular Function

identical protein binding, protein kinase binding, protein kinase C binding, protein serine/threonine kinase activity, receptor tyrosine kinase binding, SH2 domain binding, ubiquitin binding, ubiquitin protein ligase binding, zinc ion binding

Subcellular Location

Cytoplasm

Late endosome

Lysosome

Cytoplasmic vesicle › autophagosome

Nucleus

Endoplasmic reticulum

Cytoplasm › P-body

Expression

Ubiquitously expressed.

Structure

Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding.

Alternative Nomenclature

A170 

EBI 3 associated protein of 60 kDa 

EBIAP 

FTDALS3 

ORCA 

OSF-6 

Osi 

OSIL 

Oxidative stress induced like 

p60 

p62 

p62B 

Paget disease of bone 3 

PDB3 

Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa 

PKC-zeta-interacting protein 

Protein kinase C-zeta-interacting protein 

Sequestosome 1 

SQSTM 1 

STAP 

STONE14 

Ubiquitin-binding protein p62 

ZIP 

ZIP3 

General Information

Product

PRAS40 Antibody

Description

Affinity Purified Proline-rich AKT1 substrate 1 isoform a Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein.

Accession #

NCBI:  NP_115751

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96B36

Overview

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Molecular Function

negative regulation of cell size, negative regulation of protein kinase activity, negative regulation of TOR signaling, neurotrophin TRK receptor signaling pathway, regulation of cellular response to heat, regulation of neuron apoptotic process

Subcellular Location

Cytoplasm > cytosol

Expression

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Alternative Nomenclature

40 kDa proline rich AKT substrate antibody
AKT1 S1 antibody
AKT1 substrate 1 (proline rich) antibody
AKT1S1 antibody
Lobe antibody
PRAS antibody
PRAS40 antibody
Proline rich Akt substrate 40 kDa antibody
Proline-rich AKT1 substrate 1 antibody