General Information

Product

DYRK1 Antibody

Description

Affinity Purified Dual specificity tyrosine-phosphorylation-regulated kinase 1A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human Dual specificity tyrosine-phosphorylation-regulated kinase 1A.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13627

Overview

May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates such as CRY2, FOXO1, SRSF6 and SIRT1. Exhibits a sugstrate preference for proline at position P+1 and arginine at position P-3.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Nucleus speckle

Expression

Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

Structure

The polyhistidine repeats act as targeting signals to nuclear speckles.

Alternative Nomenclature

Dual specificity tyrosine phosphorylation regulated kinase
Dual specificity YAK1-related kinase
DYRK
DYRK1 
DYRKA
hMNB
HP86
Minibrain, Drosophila, homolog of
MNB
MNB protein kinase
Mnb protein kinase homolog hp86
MNB protein kinase, Serine/threonine-specific
MNB/DYRK protein kinase
MNBH
MRD7 
Protein kinase minibrain homolog
Serine/threonine kinase MNB

General Information

Product

LIMK1 Antibody

Description

Affinity Purified LIM domain kinase 1 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Accession #

NCBI:  NP_002305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53667

Overview

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Molecular Function

ATP binding, protein kinase activity, protein serine/threonine kinase activity, zinc ion binding, heat shock protein binding

Subcellular Location

Cytoplasm, nucleus

Expression

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Alternative Nomenclature

EC 2.7.11.1 antibody

LIM domain kinase 1 antibody

LIM motif-containing protein kinase antibody

LIMK antibody

LIMK-1 antibody