General Information

Product

IL12 p40 Antibody

Description

Affinity Purified Interleukin-12 subunit beta precursor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region spanning 280-335 on rat Interleukin-12 subunit beta protein.

Accession #

NCBI:  NP_072133.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

ffinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9R278

Overview

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Molecular Function

Cytokine receptor activity, protein heterodimerization activity, interleukin-12 alpha subunit binding

Alternative Nomenclature

40 kD Subunit antibody

CLMF 2 antibody

CLMF antibody

Cytotoxic lymphocyte maturation factor 2 antibody

Cytotoxic lymphocyte maturation factor 2 p40 antibody

HGNC:5970 antibody

IL 12 subunit p40 antibody

IL 12B antibody

Interleukin 12 beta chain antibody

Interleukin 12 p40 antibody

Natural killer cell stimulatory factor 2 antibody

NKSF 2 antibody

NKSF antibody

p40 antibody

TcMF antibody

TSF antibody

General Information

Product

ILK Antibody

Description

Affinity Purified Integrin-linked protein kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-452 on human Integrin-linked protein kinase.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.87 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13418

Overview

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.

Molecular Function

ATP binding, protein kinase binding, protein serine/threonine kinase activity, signal transducer activity

Subcellular Location

Cell junction, cell membrane, peripheral membrane protein, cytoplasmic side, cell projection

Expression

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Alternative Nomenclature

59 kDa serine/threonine protein kinase antibody

DKFZp686F1765 antibody

ILK 1 antibody

ILK 2 antibody

ILK antibody

Integrin linked kinase 2 antibody

Integrin-linked protein kinase antibody

p59 antibody

p59ILK antibody

General Information

Product

Lyn Antibody

Description

Affinity Purified Tyrosine-protein kinase Lyn Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 425-525 on human Lyn protein.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

MIG7 Antibody Affinity Purified

Description

Affinity Purified MIG7 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 170-220 on MIG7 protein.

Accession #

GenBank: ABF71424.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q1AHR6

Alternative Nomenclature

mig 7 antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 130-180 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 and 950-976 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody Mid-Region

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 430-480 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody Extreme C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 950-976 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

Calcitonin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 30-80 on human Calcitonin protein.

Accession #

GenBank: CAA26189.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01258

Overview

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Katacalcin is a potent plasma calcium-lowering peptide.

Molecular Function

Hormone; Calcitonin receptor binding; Identical protein binding

Subcellular Location

Secreted

Structure

Belongs to calcitonin family; Signal domain

Alternative Nomenclature

Alpha CGRP antibody

CALC1 antibody

CALCA antibody

Calcitonin related polypeptide alpha antibody

CGRP1 antibody

CT antibody

Katacalcin antibody

KC antibody