General Information

Product

PKC epsilon Antibody

Description

Affinity Purified Protein Kinase C epsilon Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Protein Kinase C epsilon type protein.

Accession #

NCBI:  NP_005391.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02156

Overview

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardio myocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAP, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implication the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaciton with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a rolei n the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). invovled in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be invovled in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin-and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. IS able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines produciton. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Cell Membrane; Nucleus

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Alternative Nomenclature

KPCE_HUMAN antibody

MGC125656 antibody

MGC125657 antibody

nPKC epsilon antibody

nPKC-epsilon antibody

PKCE antibody

Pkcea antibody

PRKCE antibody

Protein kinase C epsilon antibody

General Information

Product

Syk Antibody

Description

Affinity Purified tyrosine-protein kinase SYK Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Syk protein.

Accession #

NCBI:  NP_001167639.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P43405

Overview

Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.

Subcellular Location

Cell membrane, cytoplasm, cytosol

Expression

Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.

Alternative Nomenclature

EC 2.7.10.2 antibody

kinase Syk antibody

KSYK antibody

p72-Syk antibody

Spleen tyrosine kinase antibody

Syk antibody

Tyrosine protein kinase SYK antibody

General Information

Product

SIGLEC10 Antibody

Description

Affinity Purified Sialic acid-binding Ig-like lectin 10 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 650-697 on human SIGLEC10 protein.

Accession #

NCBI:  NP_149121.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.60 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q96LC7

Overview

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Molecular Function

carbohydrate binding

Subcellular Location

Isoform 1,2,3,4: cell membrane, single-pass type I membrane protein; isoform 5: secreted

Expression

Expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform5 is found to be the most abundant isoform. Found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells.

Alternative Nomenclature

PRO940 antibody

sialic acid binding Ig like lectin 10 Ig like lectin 7 antibody

Sialic acid binding Immunoglobulin like lectin 10 antibody

SIGLEC 10 antibody

Siglec G antibody

Siglec-like protein 2 antibody

SIGLEC10 antibody

Siglecg antibody

SLG2 antibody

General Information

Product

SRPX2 Antibody

Description

Affinity Purified Sushi repeat-containing protein SRPX2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-470 on human SRPX2 protein.

Accession #

NCBI:  NP_055282.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O60687

Overview

Acts as a ligand for the urokinase plasminogen activator surface receptor. Plays role in andiogenesis by inducing endothelial cell migration and formation of vascular network Involved in cell migration and adhesion.

Molecular Function

Hepatocyte growth factor binding, identical protein binding, receptor binding

Subcellular Location

Secreted, cytoplasm, cell surface, cell junction, synapse

Expression

Expressed in neurons of the rolandic area of the brain (at protein level). Highly expressed in the brain, placenta, lung, trachea, uterus, adrenal gland, heart, ovary and placenta. Weakly expressed in the peripheral blood, brain and bone marrow. Expressed in numerous cancer cell lines and in gastrointestinal cancer cells. Higher levels found in colorectal cancers than in normal colonic mucosa.

Alternative Nomenclature

BPP antibody

CBPS antibody

PMGX antibody

RESDX antibody

SRPUL antibody

SRPX2 antibody

Sushi repeat containing protein SRPX2 antibody

Sushi repeat containing protein X linked 2 antibody

Sushi repeat protein antibody

General Information

Product

Galectin 3 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid sequences 1-100 on human Galectin 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P17931

Overview

Galactose-specific lectin which binds IgE.  Mediates the alpha-3, beta-1 integrin the stimulation CSPG4 of endothelial cells migration.  Invovled in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Molecular Function

IgE-binding protein, Lectin

Subcellular Location

Cytoplasm; Nucleus; Secreted

Expression

Found in colonic epithelium.  Abundant in activated macrophages.  Expressed in fetal membranes.

Structure

Probably forms homodimer or heterodimers.  Forms complex with the ITGA3, ITGB1 and CSPG4.

Alternative Nomenclature

35 kDa lectin antibody

Carbohydrate-binding protein 35 antibody

CBP 35 antibody

GAL3 antibody

Galactose specific lectin 3 antibody

Galactoside-binding protein antibody

GALBP antibody

GALIG antibody

GBP antibody

IgE binding protein antibody

L 31 antibody

L 34 antibody

Laminin-binding protein antibody

Lectin galactose binding soluble 3 antibody

Lectin L-29 antibody

Lectin, galactose binding, soluble 3 antibody

LGALS 3 antibody

LGALS2 antibody

MAC 2 antigen antibody

Macrophage galactose-specific lectin antibody

General Information

Product

Galectin 3 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid sequences 150-250 on human Galectin 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P17931

Overview

Galactose-specific lectin which binds IgE.  Mediates the alpha-3, beta-1 integrin the stimulation CSPG4 of endothelial cells migration.  Invovled in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Molecular Function

IgE-binding protein, Lectin

Subcellular Location

Cytoplasm; Nucleus; Secreted

Expression

Found in colonic epithelium.  Abundant in activated macrophages.  Expressed in fetal membranes.

Structure

Probably forms homodimer or heterodimers.  Forms complex with the ITGA3, ITGB1 and CSPG4.

Alternative Nomenclature

35 kDa lectin antibody

Carbohydrate-binding protein 35 antibody

CBP 35 antibody

GAL3 antibody

Galactose specific lectin 3 antibody

Galactoside-binding protein antibody

GALBP antibody

GALIG antibody

GBP antibody

IgE binding protein antibody

L 31 antibody

L 34 antibody

Laminin-binding protein antibody

Lectin galactose binding soluble 3 antibody

Lectin L-29 antibody

Lectin, galactose binding, soluble 3 antibody

LGALS 3 antibody

LGALS2 antibody

MAC 2 antigen antibody

Macrophage galactose-specific lectin antibody

General Information

Product

CD99 Antibody

Description

Affinity Purified CD99 Antigen Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Baboon, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 50-100 to human CD99 antigen.

Accession #

GenBank: EAW98700.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI0000EE42C0

Overview

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Molecular Function

Cell Adhesion

Subcellular Location

Membrane

Structure

Belongs to CD99 family

Alternative Nomenclature

CD99 antibody

CD99 antigen antibody

CD99 molecule antibody

CD99X antibody

E2 antigen antibody

Ewing Sarcoma antibody

MIC 2X antibody

MIC 2Y antibody

MIC2 antibody

MSK5X antibody

Protein MIC2 antibody

Surface antigen MIC2 antibody

T cell surface glycoprotein E2 antibody

General Information

Product

CD99 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 97-147 to human CD99 antigen.

Accession #

GenBank: EAW98700.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI0000EE42C0

Overview

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Molecular Function

Cell Adhesion

Subcellular Location

Membrane

Structure

Belongs to CD99 family

Alternative Nomenclature

12E7 antibody

CD99 antibody

CD99 antigen antibody

CD99 molecule antibody

CD99X antibody

E2 antigen antibody

Ewing Sarcoma antibody

MIC 2X antibody

MIC 2Y antibody

MIC2 antibody

MSK5X antibody

Protein MIC2 antibody

Surface antigen MIC2 antibody

T cell surface glycoprotein E2 antibody

General Information

Product

Bestrophin Antibody

Description

Affinity Purified Bestrophin 1 Antibody N-epitope

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope Synthetic peptide corresponding to unique amino acid sequences on human Bestrophin 1 protein.

Accession #

NCBI: NP_004174.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O76090

Overview

Forms calcium sensitive chloride channels. Highly permeable to bicarbonate.

Molecular Function

Ion transport; Sensory transduction; Transport; Vision

Subcellular Location

Cell membrane; Multi-pass membrane protein; Basolateral cell membrane

Expression

Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.

Structure

Tetramer or pentamers

Alternative Nomenclature

ARB antibody

BEST 1 antibody

BEST antibody

Best disease antibody

BEST1 antibody

Bestrophin1 antibody

BMD antibody

mBest1 antibody

RP50 antibody

TU15B antibody

Vitelliform macular dystrophy 2 antibody

VMD2 antibody

General Information

Product

Bestrophin Antibody

Description

Affinity Purified Bestrophin 1 Antibody Mid-Region

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Mid-Region Synthetic peptide corresponding to unique amino acid sequences on human Bestrophin 1 protein.

Accession #

NCBI: NP_004174.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DOT Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O76090

Overview

Forms calcium sensitive chloride channels. Highly permeable to bicarbonate.

Molecular Function

Ion transport; Sensory transduction; Transport; Vision

Subcellular Location

Cell membrane; Multi-pass membrane protein; Basolateral cell membrane

Expression

Predominantly expressed in the basolateral membrane of the retinal pigment epithelium

Structure

Tetramer or pentamers

Alternative Nomenclature

ARB antibody

BEST 1 antibody

BEST antibody

Best disease antibody

BEST1 antibody

Bestrophin1 antibody

BMD antibody

mBest1 antibody

RP50 antibody

TU15B antibody

Vitelliform macular dystrophy 2 antibody

VMD2 antibody