General Information

Product

Bcl6 Antibody

Description

Affinity Purified B-Cell lymphoma 6 protein Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 610-660 on human B-cell lymphoma 6 protein isoform 1.

Accession #

NCBI:  NP_001124317

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P41182

Overview

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Molecular Function

Chromatin Binding; Actin Cytoskeleton organization; Cellular response to DNA damage stimulus; Negative regulation of cell growth; Activator; Repressor

Subcellular Location

Nucleus

Expression

Expressed in germinal center T- and B-cells and in primary immature dendritic cells.

Structure

Homodimer.

Alternative Nomenclature

B cell CLL/lymphoma 6 antibody

B cell lymphoma 6 protein antibody

B-cell lymphoma 5 protein antibody

BCL5 antibody

BCL6A antibody

cys his2 zinc finger transcription factor antibody

LAZ3 antibody

Lymphoma associated zinc finger gene on chromosome 3 antibody

Protein LAZ-3 antibody

ZBTB27 antibody

Zinc finger and BTB domain-containing protein 27 antibody

Zinc finger protein 51 antibody

zinc finger transcription factor BCL6S antibody

ZNF 51 antibody

General Information

Product

Bcl6 Antibody

Description

Affinity Purified B-Cell lymphoma 6 protein Extreme C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 650-700 on human B-cell lymphoma 6 protein isoform 1.

Accession #

NCBI:  NP_001124317

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P41182

Overview

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Molecular Function

Chromatin Binding; Actin Cytoskeleton organization; Cellular response to DNA damage stimulus; Negative regulation of cell growth; Activator; Repressor

Subcellular Location

Nucleus

Expression

Expressed in germinal center T- and B-cells and in primary immature dendritic cells.

Structure

Homodimer.

Alternative Nomenclature

B cell CLL/lymphoma 6 antibody

B cell lymphoma 6 protein antibody

B-cell lymphoma 5 protein antibody

BCL5 antibody

BCL6A antibody

cys his2 zinc finger transcription factor antibody

LAZ3 antibody

Lymphoma associated zinc finger gene on chromosome 3 antibody

Protein LAZ-3 antibody

ZBTB27 antibody

Zinc finger and BTB domain-containing protein 27 antibody

Zinc finger protein 51 antibody

zinc finger transcription factor BCL6S antibody

ZNF 51 antibody

General Information

Product

C-Myc Antibody

Description

Affinity Purified myc proto-oncogene protein Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human cMyc protein.

Accession #

NCBI:  NP_002458

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01106

Overview

Transcription factor that binds DNA in non-specific manner.  Also specifically recognizes the core sequence 5'CAC[GA]TG-3'.  Activates transcription of growth-related genes.

Molecular Function

Activator; Transcription regulation; DNA-binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Myc proto oncogene protein antibody

myc-related translation/localization regulatory factor antibody

MYCC antibody

Myelocytomatosis oncogene antibody

Niard antibody

Nird antibody

Oncogene Myc antibody

Proto-oncogene c-Myc antibody

Protooncogene homologous to myelocytomatosis virus antibody

RNCMYC antibody

Transcription factor p64 antibody

Transcriptional regulator Myc-A antibody

V-Myc avian myelocytomatosis viral oncogene homolog antibody

General Information

Product

C-Myc Antibody Affinity Purified

Description

Affinity Purified myc proto-oncogene protein Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 400-454 on human cMyc protein.

Accession #

NCBI:  NP_002458

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01106

Overview

Transcription factor that binds DNA in non-specific manner.  Also specifically recognizes the core sequence 5'CAC[GA]TG-3'.  Activates transcription of growth-related genes.

Molecular Function

Activator; Transcription regulation; DNA-binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Myc proto oncogene protein antibody

myc-related translation/localization regulatory factor antibody

MYCC antibody

Myelocytomatosis oncogene antibody

Niard antibody

Nird antibody

Oncogene Myc antibody

Proto-oncogene c-Myc antibody

Protooncogene homologous to myelocytomatosis virus antibody

RNCMYC antibody

Transcription factor p64 antibody

Transcriptional regulator Myc-A antibody

V-Myc avian myelocytomatosis viral oncogene homolog antibody

General Information

Product

ergo-1 Antibody 

Description

Affinity Purified Endogenous-RNAi deficient arGOnaute Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 190-240 on C.elegans Endogenous-RNAi deficient arGOnaute.

Accession #

NCBI:  NP_503362.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O61931

Overview

Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Interacts with 26G small interfering RNAs (26G-siRNAs) and required for subsequent biogenesis of secondary 22G-siRNAs. May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed: 22102828).

Molecular Function

Dead/H-box RNA helicase binding, nucleic acid binding, production of siRNA involved in RNA interference, regulation of translation, RNA interference.

Subcellular Location

Cytoplasm

Structure

Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.

Alternative Nomenclature

Endogenous RNAi deficient argonaute antibody

Ergo 1 antibody

Hypothetical protein R09A1.1 antibody

General Information

Product

ergo-1 Antibody

Description

Affinity Purified Endogenous-RNAi deficient arGOnaute antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 790-840 on C.elegans Endogenous-RNAi deficient arGOnaute protein.

Accession #

NCBI:  NP_503362.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O61931

Overview

Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Interacts with 26G small interfering RNAs (26G-siRNAs) and required for subsequent biogenesis of secondary 22G-siRNAs. May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed: 22102828).

Molecular Function

Dead/H-box RNA helicase binding, nucleic acid binding, production of siRNA involved in RNA interference, regulation of translation, RNA interference.

Subcellular Location

Cytoplasm

Structure

Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.

Alternative Nomenclature

Endogenous RNAi deficient argonaute antibody

Ergo 1 antibody

Hypothetical protein R09A1.1 antibody

General Information

Product

CDX2 Antibody Affinity Purified

Description

Affinity Purified Homeobox Protein CDX2 Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 1-50 on human CDX2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q99626

Overview

Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

Molecular Function

Activator, Developmental Protein

Subcellular Location

Nucleus

Structure

Belongs to the Caudal homeobox family; Contains 1 homeobox DNA-binding domain

Alternative Nomenclature

Caudal type homeo box 2 antibody

Caudal type homeo box transcription factor 2 antibody

CDX 2 antibody

CDX 3 antibody

Homeobox protein CDX2 antibody

General Information

Product

CDX2 Antibody

Description

Affinity Purified Homeobox Protein CDX2 Antibody Mid-Region

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Mid-Region epitope synthetic peptide taken within amino acid region 100-200 on human CDX2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q99626

Overview

Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

Molecular Function

Activator, Developmental Protein

Subcellular Location

Nucleus

Structure

Belongs to the Caudal homeobox family; Contains 1 homeobox DNA-binding domain

Alternative Nomenclature

Caudal type homeo box 2 antibody

Caudal type homeo box transcription factor 2 antibody

CDX 2 antibody

CDX 3 antibody

Homeobox protein CDX2 antibody

General Information

Product

Dfos Antibody

Description

Affinity Purified Drosophila dFRA mRNA for Fos-related antigen Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 5-80 on DFos protein.

Accession #

GenBank: X54143.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P21525

Overview

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'/  May play a role in the function or determination of a particular subset of cells in the developing embryo.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Expression

Early expression in mesodermal and cephalic furrow.  Late embryonic development expression is ectodermal, corresponding to muscle attachment sites.  Also in midgut, hindgut and analpad.

Structure

Homodimer; Heterodimer with Jra

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

G0 G1 switch regulatory protein 7 antibody

G0S7 antibody

Oncogene FOS antibody

p55 antibody

proto oncogene c Fos antibody

v fos FBJ murine osteosarcoma viral oncogene homolog antibody

General Information

Product

Dfos Antibody

Description

Affinity Purified Drosophila dFRA mRNA for Fos-related antigen Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 450-550 on DFos protein.

Accession #

GenBank: X54143.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P21525

Overview

Developmentally regulated transcription factor AP-1 binds and recognizes the enhancer DNA sequence: 5'-TGA[CG]TCA-3'/  May play a role in the function or determination of a particular subset of cells in the developing embryo.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Expression

Early expression in mesodermal and cephalic furrow.  Late embryonic development expression is ectodermal, corresponding to muscle attachment sites.  Also in midgut, hindgut and analpad.

Structure

Homodimer; Heterodimer with Jra

Alternative Nomenclature

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

G0 G1 switch regulatory protein 7 antibody

G0S7 antibody

Oncogene FOS antibody

p55 antibody

proto oncogene c Fos antibody

v fos FBJ murine osteosarcoma viral oncogene homolog antibody