StripOBufferTM is a specially formulated water based buffer that will strip antibodies from antigens in 5-10 minutes at room temperature
Room temperature for short-term storage
25 mL of StripObuffer can be used on 25-50 mini-blots.
Save time by re-using blots
Preserve protein and antigen samples
Eliminate issues with proper equal protein loading
Compare phosphorylated and non-phosphorylated state of the same protein
on the same blot
Reconfirm protein expression by re-probing the same blot with different set of
antibodies raised against antibodies from different vendors or same or different epitopes
Preform multiple stripping’s while maintaining antigenicity
No heating required
No β-mercaptoethanol required – No smell
Compatible with Nitrocellulose and PVDF membranes
Dilute (1:10) the supplied buffer in distilled water prior to use.
Rinse the membrane in distilled water first and then in stripping buffer at least once.
Multiple rinses with buffer results in clearer bands on autoradiograms
Immerse the membrane completely in 10 mL of stripping buffer for each mini-gel membrane.
Gently shake the membrane on a rocker for 5-10 minutes at room temperature.
Rinse the membrane with distilled water.
Rinse the membrane with TBST or the buffer in which your primary antibody is diluted.
Proceed for blocking stage.
Fabgennix StripOBuffer has been used in 2 Publications
Gebska, Milena A. et al. “Phosphodiesterase-5A (PDE5A) Is Localized to the Endothelial Caveolae and Modulates NOS3 Activity.”Cardiovascular Research90.2 (2011): 353–363.PMC3078797
Pawar, Vaibhav et al. “Checkpoint Kinase Phosphorylation in Response to Endogenous Oxidative DNA Damage in Repair-Deficient Stationary-PhaseSaccharomyces Cerevisiae.”Mechanisms of ageing and development130.8 (2009): 501–508.PMC2716406
Publishing research using StripOBuffer? Pleaselet us know, we can cite the reference in this datasheet.