PARD6B Antibody

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 340- 372 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody