IRE1A Positive Control

General Information

Product

IRE1A Positive Control

Description

Western Blot Positive Control Serine/Threonine-protein kinase/endoribonuclease IRE1

Verified Applications

WB

Immunogen

Purified Protein

Physical Properties

Quantity

5 Applications

Volume

Inquire

Immunoglobulin

IgG

Form

Western Blot Positive Control

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

O75460

Overview

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Molecular Function

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein

Expression

Ubiquitously expressed. High levels observed in pancreatic tissue.

Structure

Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.

Alternative Nomenclature

Endoplasmic reticulum (ER) to nucleus signalling 1 antibody Endoribonuclease antibody
ER to nucleus signaling 1 antibody

ERN 1 antibody

hIRE 1p antibody
Inositol requiring 1 antibody
Inositol requiring protein 1 antibody
IRE 1 antibody
IRE 1a antibody
IRE 1P antibody
Ire1 alpha antibody

IRE1P antibody
MGC163277 antibody
MGC163279 antibody
Protein kinase/endoribonuclease antibody
RGD1559716 antibody
Serine/threonine protein kinase/endoribonuclease IRE1 antibody