Src Antibody

General Information

Product

Src Antibody

Description

Proto-oncogene tyrosine-protein kinase Src Antibody C-epitope

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-500 on human Src protein.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.60 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation.

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

c SRC antibody

CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

EC 2.7.10.2 antibody

Neuronal CSRC tyrosine specific protein kinase antibody

Neuronal SRC antibody

Oncogene SRC antibody

p60 Src antibody

Proto-oncogene tyrosine-protein kinase Src antibody

SRC proto oncogene non receptor tyrosine kinase antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

Tyrosine protein kinase SRC1 antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody