General Information

Product

RRF3 Antibody Affinity Purified

Description

Affinity Purified RNA Dependent RNA Polymerase family member 3 Antibody Mid-Region

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 1355-1405 on C. elegans RRF-3 protein.

Accession #

NCBI:  NP_495713.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

G5EE53

Overview

RNA-directed RNA polymerase activity

Alternative Nomenclature

RNA polymerase family member 3  antibody RNA polymerase III

General Information

Product

RRF3 Antibody Affinity Purified

Description

Affinity Purified RNA Dependent RNA Polymerase family member 3 Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 1715-1765 on C. elegans RRF-3 protein.

Accession #

NCBI:  NP_495713.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

G5EE53

Overview

RNA-directed RNA polymerase activity

Alternative Nomenclature

RNA polymerase family member 3  antibody RNA polymerase III

General Information

Product

RRF3 Antibody Affinity Purified

Description

Affinity Purified RNA Dependent RNA Polymerase family member 3 Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 1320-1370 on C. elegans RRF-3 protein.

Accession #

NCBI:  NP_495713.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

G5EE53

Overview

RNA-directed RNA polymerase activity

Alternative Nomenclature

RNA polymerase family member 3  antibody RNA polymerase III

General Information

Product

TIA1 Antibody

Description

Affinity Purified Nucleolysin TIA-1 isoform p40 Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 414-464 on human TIA1 protein.

Accession #

GenBank: BAD92448.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50

Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:500

Protein

Uniprot #

P31483

Overview

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Molecular Function

AU-rich element binding, nucleotide binding, poly(A) binding, poly (A) RNA binding

Subcellular Location

Cytoplasmic granule, nucleus. (Note: accumulates in cytoplasmic stress granules following cellular damage

Alternative Nomenclature

Cytotoxic granule associated RNA binding protein 1 antibody

Nucleolysin TIA 1 isoform p40 antibody

p40 TIA 1 antibody

p40-TIA-1 (containing p15-TIA-1) antibody

RNA binding protein TIA 1 antibody

T-cell-restricted intracellular antigen-1 antibody

TIA 1 cytotoxic granule associated RNA binding protein antibody

TIA1 cytotoxic granule associated RNA binding protein like 1 antibody

TIA1 protein antibody

TIAL1 antibody

TIAR antibody

General Information

Product

TIA1 Antibody Affinity Purified

Description

Affinity Purified Nucleolysin TIA-1 isoform p40 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human TIA1 protein.

Accession #

GenBank: BAD92448.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50

Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:500

Protein

Uniprot #

P31483

Overview

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Molecular Function

AU-rich element binding, nucleotide binding, poly(A) binding, poly (A) RNA binding

Subcellular Location

Cytoplasmic granule, nucleus. (Note: accumulates in cytoplasmic stress granules following cellular damage

Alternative Nomenclature

Cytotoxic granule associated RNA binding protein 1 antibody

Nucleolysin TIA 1 isoform p40 antibody

p40 TIA 1 antibody

p40-TIA-1 (containing p15-TIA-1) antibody

RNA binding protein TIA 1 antibody

T-cell-restricted intracellular antigen-1 antibody

TIA 1 cytotoxic granule associated RNA binding protein antibody

TIA1 cytotoxic granule associated RNA binding protein like 1 antibody

TIA1 protein antibody

TIAL1 antibody

TIAR antibody

General Information

Product

GCN2 Antibody

Description

Affinity Purified eIF2alpha kinase Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1600-1700 on human GCN2 protein.

Accession #

GenBank:  CAB58363.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9P2K8

Overview

Can phosphorylate alpha subunit of EIF2 and mediates translational control.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Cytosolic ribosome

Expression

Liver, brain.

Structure

Contains 2 protein kinase domains; Contains 1 RWD domain.

Alternative Nomenclature

Eif2ak4 antibody

Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody

GCN2 antibody

GCN2 eIF2alpha kinase antibody

GCN2 like protein antibody

KIAA1338 antibody

MGCN2 antibody

General Information

Product

TdT Antibody

Description

Affinity Purified DNA nucleotidylexotransferase Antibody Extreme C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 459-509 on human Terminal deoxynucleotidyltransferase protein.

Accession #

NCBI:  NP_004079

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:1,000

Protein

Uniprot #

P04053

Overview

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Molecular Function

Nucleotidyltransferase, Transferase, DNA binding

Subcellular Location

Nucleus

Structure

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.

Alternative Nomenclature

Deoxynucleotidyltransferase terminal antibody
DNA nucleotidylexotransferase antibody
DNTT antibody
Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
TDT antibody
Terminal addition enzyme antibody
Terminal deoxynucleotidyltransferase antibody
Terminal transferase antibody

General Information

Product

TdT Antibody

Description

Affinity Purified DNA nucleotidylexotransferase Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Terminal deoxynucleotidyltransferase protein.

Accession #

NCBI:  NP_004079

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:1,000. Detects a band of approximately 58 kDa.

Protein

Uniprot #

P04053

Overview

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Molecular Function

Nucleotidyltransferase, Transferase, DNA binding

Subcellular Location

Nucleus

Structure

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.

Alternative Nomenclature

Deoxynucleotidyltransferase terminal antibody
DNA nucleotidylexotransferase antibody
DNTT antibody
Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
TDT antibody
Terminal addition enzyme antibody
Terminal deoxynucleotidyltransferase antibody
Terminal transferase antibody

General Information

Product

Cytokeratin 7 Antibody

Description

keratin, type II cytoskeletal 7 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human keratin, type II cytoskeletal 7 protein.

Accession #

NCBI:  NP_005547

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

P08729.5

Overview

Serves to block interferon-dependent interphase and stimulates DNA synthesis.  Plays a role in the translational regulation of human papillomavirus type 16 E7 mRNA.

Molecular Function

Structural molelcule activity; Host-virus interaction

Subcellular Location

Cytoplasm

Expression

Expressed in cultured epidermal, bronchial and mesothelial cells.  Seen throughout the glandular cells at the junction of the stomac and esophagus; not present in the esophogus, colon, ectocervix and liver.

Structure

Heterotetramer of two type I and two type II keratins, interacts with eukaryotic translation initiator factor 3 subunit EIF3S10 , HPV16 E7, and GPER1.

Alternative Nomenclature

CK 7 antibody

Cytokeratin 7 antibody

D15Wsu77e antibody

K2C7 antibody

K7 antibody

Keratin 55k type ii cytoskeletal antibody

Keratin 7 antibody

Keratin simple epithelial type 1 k7 antibody

Keratin type II cytoskeletal 7 antibody

Keratin-7 antibody

KRT 7 antibody

Krt2-7 antibody

MGC11625 antibody

MGC129731 antibody

mgc3625 antibody

Sarcolectin antibody

SCL antibody

Type ii mesothelial keratin k7 antibody

Type-II keratin Kb7 antibody

General Information

Product

ZFP36L2 Antibody

Description

Affinity Purified Zinc finger protein 36, C3H1 type-like 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid reigon 350-400 on human Zinc finger protein 36, C3H1 type-like 2.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:150-1:500

Protein

Uniprot #

P47974

Overview

mRNA-binding protein that plays a key role in self-renewal of erythroid cells in response to glucocorticoids. Specifically binds to the AU-rich element (ARE) in the 3'-UTR of target mRNAs, promoting their deadenylation and degradation (PubMed:14981510). Specifically expressed in burst-forming unit-erythroid (BFU-E) progenitors in response to glucocorticoids and acts as a negative regulator of erythroid cell differentiation: promotes self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. Down-regulated during erythroid differentiation from the BFU-E stage, stabilizing mRNAs required for terminal differentiation.

Molecular Function

AU-rich element binding, DNA binding, metal ion binding, mRNA 3'-UTR AU-rich region binding, poly(A) RNA binding, transcription factor activity, sequence-specific DNA binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Repeat, Zinc-finger

Alternative Nomenclature

BRF2 antibody

Butyrate response factor 2 antibody

C3H1 type-like 2 antibody

EGF response factor 2 antibody

ERF 2 antibody

Protein TIS11D antibody

RNF162C antibody

TIS11D antibody

ZFP36 like 2 antibody

ZFP36 ring finger protein like 2 antibody

Zinc finger protein 36 antibody

Zinc finger protein 36, C3H type like 1 antibody

zinc finger protein 36, C3H type like 2 antibody