General Information

Product

Integrin beta 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Integrin Beta 3 subunit protein.

Accession #

GenBank: AAB23689.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P05106

Overview

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Molecular Function

Cell adhesion, Host-virus interaction

Subcellular Location

Cell junction, Cell membrane, Cell projection, Membrane

Expression

Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Structure

Heterodimer of an alpha and a beta subunit. Beta-3 (ITGB3) associates with either alpha-IIb (ITGA2B) or alpha-V (ITGAV). Isoform Beta-3C interacts with FLNB. Interacts with COMP. Interacts with PDIA6 following platelet stimulation. Interacts with SYK; upon activation by ITGB3 promotes platelet adhesion. Interacts with MYO10. Interacts with DAB3. Interacts with FERMT2. Interacts with EMP2; regulates the levels of the heterodimer ITGA5:ITGB3 integrin expression on the plasma membrane (PubMed:16216233). Integrin ITGAV:ITGB3 interacts with FBLN5 (via N-terminus). Integrin ITGAV:ITGB3 interacts with herpes virus 8/HHV-8 glycoprotein B and acts as a receptor for the virus. Interacts with HIV-1 Tat.

Alternative Nomenclature

BDPLT16 antibody

BDPLT2 antibody

CD61 antibody

GP3A antibody

GT antibody

HPA 1 antibody

HPA 4 antibody

Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody

Integrin beta chain beta 3 antibody

ITGB 3 antibody

NAIT antibody

Platelet fibrinogen receptor, beta subunit antibody

Platelet glycoprotein IIIa antibody

Platelet membrane glycoprotein IIIa antibody

PTP antibody

General Information

Product

MRP1 Antibody

Description

Affinity Purified Multidrug resistance-associated protein 1 Antibody

Verified Applications

ELISA, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on rat multidrug resistance-associated protein 1.

Accession #

NCBI:  NP_071617.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8CG09

Overview

Mediates export of organic anions and drugs from the cytoplasm.

Molecular Function

Hydrolase

Subcellular Location

Cell membrane, Membrane

Expression

Skeletal muscle, brain, heart, spleen, lung and kidney.

Structure

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Alternative Nomenclature

ABC29 antibody

ABCC antibody

ABCC1 antibody

ATP binding cassette sub family C (CFTR/MRP) member 1 antibody

ATP binding cassette sub-family C member 1 antibody

DKFZ antibody

General Information

Product

SIRT3 Antibody

Description

Affinity Purified NAD-dependent protein deacetylase sirtuin-3, mitochondrial Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide:

RDLVGPFVLSPRRKDVVQL

corresponding to amino acids 203-221 of rat SIRT3 protein.

Accession #

NCBI:  NP_001099783

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B2RZ31

Molecular Function

enzyme binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K14 specific)

Alternative Nomenclature

Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody

NAD dependent deacetylase sirtuin 3 mitochondrial antibody

Regulatory protein SIR2 homolog 3 antibody

Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody

SIR 2 like protein 3 antibody

SIR 3 antibody

SIR2 L3 antibody

Sirtuin 3 antibody

Sirtuin type 3 antibody

General Information

Product

SIRT7 Antibody

Description

Affinity Purified Sirtuin (silent mating type information regulation 2 homolog) 7 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on mouse SIRT7 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8BKJ9

Overview

NAD-dependent protein deacetylase that specifially mediates deacetylation of histone H3 at 'Lys-18'. Displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression.

Molecular Function

Chromatin binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K18 specific), metal ion binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: Located close to the nuclear membrane when in cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Expression

Detected in liver, spleen and testis. Detected in embryos.

Structure

Belongs to the Sirtuin Family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIRT7 antibody

Sirtuin 7 antibody

Sirtuin type 7 antibody

General Information

Product

Gastrin Antibody Affinity Purified

Description

Affinity Purified Gastrin Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Gastrin preprotein.

Accession #

NCBI:  NP_000796.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01350

Overview

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.

Molecular Function

Hormone Activity

Subcellular Location

Secreted

Expression

Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.

Structure

Belongs to the gastrin/cholecytosokinin family

Alternative Nomenclature

Component II antibody

G14 antibody

G17 antibody

G34 antibody

G52 antibody

G6 antibody

GAS antibody

GAST antibody

gastrin antibody

Gastrin-14 antibody

Gastrin-17 antibody

Gastrin-34 antibody

Gastrin-52 antibody

Gastrin-6 antibody

Gastrin-71 antibody

General Information

Product

Gastrin Antibody Affinity Purified

Description

Affinity Purified Gastrin Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human Gastrin preprotein.

Accession #

NCBI:  NP_000796.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50-1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01350

Overview

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.

Molecular Function

Hormone Activity

Subcellular Location

Secreted

Expression

Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.

Structure

Belongs to the gastrin/cholecytosokinin family

Alternative Nomenclature

Component II antibody

G14 antibody

G17 antibody

G34 antibody

G52 antibody

G6 antibody

GAS antibody

GAST antibody

gastrin antibody

Gastrin-14 antibody

Gastrin-17 antibody

Gastrin-34 antibody

Gastrin-52 antibody

Gastrin-6 antibody

Gastrin-71 antibody

General Information

Product

Phospholipase A2 Antibody

Description

Affinity Purified Phospholipase A2-activating protein Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Phospholipase A2-activating protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9Y263

Overview

Involved in the maintenance of ubiquitin levels.

Molecular Function

Phospholipase A2 activator activity; Inflammatory response; Phospholipid metabolic process; Signal transduction

Subcellular Location

Extracellular vesicular exosome

Structure

Interacts with ubiquitin. Interacts with VCP.

Alternative Nomenclature

Calcium dependent phospholipid binding protein antibody

CPLA 2 antibody

Cytosolic phospholipase A2 antibody

Cytosolic phospholipase A2 group IVA antibody

Lysophospholipase antibody

MGC126350 antibody

Phosphatidylcholine 2 acylhydrolase antibody

Phospholipase A2 group 4 A antibody

Phospholipase A2 group IVA antibody

PhospholipaseA2 antibody

PLA2G4 antibody

General Information

Product

Phospholipase A2 Antibody 

Description

Affinity Purified Phospholipase A2-activating protein Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Rat, Mouse, Human, Monkey, Bovine

Immunogen

Synthetic peptide taken within amino acid region 30-80 on human Phospholipase A2-activating protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9Y263

Overview

Involved in the maintenance of ubiquitin levels.

Molecular Function

Phospholipase A2 activator activity; Inflammatory response; Phospholipid metabolic process; Signal transduction

Subcellular Location

Extracellular vesicular exosome

Structure

Interacts with ubiquitin. Interacts with VCP.

Alternative Nomenclature

Calcium dependent phospholipid binding protein antibody

CPLA 2 antibody

Cytosolic phospholipase A2 antibody

Cytosolic phospholipase A2 group IVA antibody

Lysophospholipase antibody

MGC126350 antibody

Phosphatidylcholine 2 acylhydrolase antibody

Phospholipase A2 group 4 A antibody

Phospholipase A2 group IVA antibody

PhospholipaseA2 antibody

PLA2G4 antibody

General Information

Product

beta Catenin Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 50-100 on human beta CTNNB protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P35222 

Overview

Major downstream component of the canonical Wnt signalling pathway.  Also involved in the regulation of cell adhesion and acts as a negative regulator of centrosome cohesion.

Molecular Function

Activator; Alpha-catenin binding; Cadherin binding; signal transducer activity

Subcellular Location

Cytoplasm; Nucleus; Cell junction; Cell Membrane

Expression

Expressed in hair follicle cell types including basal and peripheral matrix cells as well as cells of the outer and inner root sheaths.  Also expressed in colon and is present in cortical neurons at protein level.

Structure

Two separate complex associated pools found in cytoplasm, majority as compenent of an E-cadheren/catenin adhesion complex.  Another cytoplasmic pool is a part of a large complex which holds AXIN1, AXIN2, APC, CSNK1A1 and GSK3B

Alternative Nomenclature

Cadherin associated protein antibody

Catenin (cadherin associated protein), beta 1, 88kDa antibody

CATNB antibody

CHBCAT antibody

General Information

Product

beta Catenin Antibody Affinity Purified

Description

Affinity Purified Beta Catenin Antibody

C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 740-790 on human beta CTNNB protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P35222

Overview

Major downstream component of the canonical Wnt signalling pathway.  Also involved in the regulation of cell adhesion and acts as a negative regulator of centrosome cohesion.

Molecular Function

Activator; Alpha-catenin binding; Cadherin binding; signal transducer activity

Subcellular Location

Cytoplasm; Nucleus; Cell junction; Cell Membrane

Expression

Expressed in hair follicle cell types including basal and peripheral matrix cells as well as cells of the outer and inner root sheaths.  Also expressed in colon and is present in cortical neurons at protein level.

Structure

Two separate complex associated pools found in cytoplasm, majority as compenent of an E-cadheren/catenin adhesion complex.  Another cytoplasmic pool is a part of a large complex which holds AXIN1, AXIN2, APC, CSNK1A1 and GSK3B

Alternative Nomenclature

Cadherin associated protein antibody

Catenin (cadherin associated protein), beta 1, 88kDa antibody

CATNB antibody

CHBCAT antibody