General Information

Product

THAP1 Antibody

Description

Affinity Purified THAP domain-containing protein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-210 on human THAP domain-containing protein 1.

Accession #

GenBank: CAG33537

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NVV9

Overview

DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.

Molecular Function

Identical protein binding, Sequence-specific DNA binding, Zinc ion binding

Subcellular Location

Nucleus > nucleoplasm, PML body

Expression

Highly expressed in heart, skeletal muscle, kidney and liver. Weaker expression in brain and placenta.

Structure

Belongs to the THAP1 family.

Alternative Nomenclature

Nuclear proapoptotic factor antibody

THAP 1 antibody

THAP domain containing apoptosis associated protein 1 antibody

THAP domain containing protein 1 antibody

THAP domain protein 1 antibody

General Information

Product

Aurora A Antibody

Description

Affinity Purified Aurora Kinase A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-397 on human Aurora Kinase A protein.

Accession #

NCBI:  NP_695208

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q3MHU2

Overview

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes.

Molecular Function

ATP binding

histone serine kinase activity

protein heterodimerization activity

protein kinase activity

protein kinase binding

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

ubiquitin protein ligase binding

Subcellular Location

centrosome 

spindle pole 

cilium basal body

centriole

Expression

Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Alternative Nomenclature

AIK antibody

ARK1 antibody

AURA antibody

Aurora 2 antibody

Aurora A antibody

Aurora kinase A antibody

Aurora-related kinase 1 antibody

Aurora/IPL1 like kinase antibody

AURORA2 antibody

Breast tumor-amplified kinase antibody

BTAK antibody

hARK1 antibody

IAK antibody

IPL1 related kinase antibody

PPP1R47 antibody

Protein phosphatase 1, regulatory subunit 47 antibody

Serine/threonine kinase 15 antibody

Serine/threonine kinase 6 antibody

Serine/threonine-protein kinase aurora-A antibody

STK15 antibody

STK6 antibody

STK7 antibody

General Information

Product

Aurora B Antibody

Description

Aurora Kinase B Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 300-344 on human Aurora Kinase B protein.

Accession #

NCBI:  NP_004208.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q96GD4

Overview

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.

Molecular Function

ATP binding

histone serine kinase activity

kinase binding

metal ion binding

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

Subcellular Location

Spindle, Nucleus

Expression

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Alternative Nomenclature

AIK2 antibody

AIM1 antibody

ARK2 antibody

AurB antibody

AURKB antibody

Aurora 1 antibody

Aurora and Ipl1 like midbody associated protein 1 antibody

Aurora kinase B antibody

Aurora related kinase 2 antibody

Aurora- and Ipl1-like midbody-associated protein 1 antibody

Aurora-B antibody

Aurora/IPL1 related kinase 2 antibody

IPL1 antibody

PPP1R48 antibody

Protein phosphatase 1 regulatory subunit 48 antibody

Serine/threonine-protein kinase 12 antibody

Serine/threonine-protein kinase aurora-B antibody

STK1 antibody

STK12 antibody

STK5 antibody

General Information

Product

GPS2 Antibody

Description

Affinity Purified G protein pathway suppressor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human G protein pathway suppressor 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q13227

Molecular Function

cyclin binding

GTPase inhibitor activity

transcription coactivator activity

transcription corepressor activity

Subcellular Location

Nucleus, Cytosol

Expression

Widely expressed.

Structure

Coiled coil

Alternative Nomenclature

AMF 1 antibody

G protein pathway suppressor 2 antibody

GPS 2 antibody

General Information

Product

KMT6 / EZH2 Antibody

Description

Affinity Purified Histone-lysine N-methyltransferase EZH2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-110 on human Histone-lysine N-methyltransferase EZH protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

A0A090N8E9

Molecular Function

chromatin DNA binding

histone-lysine N-methyltransferase activity

primary miRNA binding

ribonucleoprotein complex binding

RNA polymerase II core promoter sequence-specific DNA binding

RNA polymerase II proximal promoter sequence-specific DNA binding

Subcellular Location

Nucleus, Cytoplasm

Alternative Nomenclature

Enhancer of zeste 2 antibody

enhancer of zeste 2 polycomb repressive complex 2 subunit antibody

ENX 1 antibody

Enx1h antibody

EZH 2 antibody

EZH1 antibody

EZH2b antibody

Histone-lysine N-methyltransferase EZH2 antibody

KMT 6 antibody

KMT6A antibody

Lysine N-methyltransferase 6 antibody

WVS antibody

WVS2 antibody