General Information

Product

Eprhin Receptor B4 Antibody

Description

Affinity Purified Ephrin type-B receptor 4 precursor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 900-984 on human Ephrin B4 receptor protein.

Accession #

NCBI:  NP_004435

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54760

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell Membrane

Expression

Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.

Alternative Nomenclature

Ephb4 antibody

Ephrin type-B receptor 4 antibody

Hepatoma Transmembrane Kinase antibody

HTK antibody

MYK 1 antibody

MYK1 antibody

TYRO 11 antibody

Tyrosine-protein kinase receptor HTK antibody

Tyrosine-protein kinase TYRO11 antibody

General Information

Product

Eprhin Receptor B3 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 3 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on EphBR3 protein.

Accession #

NCBI:  NP_034273

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

P54754

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level).

Structure

Contains 1 Eph LBD domain; Contains 2 fibronectin type-III domains; Contains 1 protein kinase domain; Contains 1 SAM domain

Alternative Nomenclature

Cek10 antibody

EK2 antibody

Embryonic kinase 2 antibody

EPH Like Tyrosine Kinase 2 antibody

EPH receptor B3 antibody

EPH-like kinase 2 antibody

ephb3 antibody

Ephrin type-B receptor 3 antibody

ETK2 antibody

hEK2 antibody

Human Embryo Kinase 2 antibody

Mdk5 antibody

Sek4 antibody

TYRO6 antibody

Tyrosine protein kinase receptor HEK2 antibody

Tyrosine-protein kinase TYRO6 antibody

General Information

Product

Eprhin Receptor A8 Antibody Affinity Purified

Description

Affinity Purified Ephrin Type-A receptor 8 isoform 1 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on human EphA R8 protein.

Accession #

NCBI:  NP_065387

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein; Early endosome membrane

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. May also form heterodimers with other ephrin receptors (By similarity).

Alternative Nomenclature

2.7.1.112 antibody

EEK antibody

EK3 antibody

EPH and ELK related kinase antibody

EPH like kinase 3 antibody

EphA8 antibody

Ephrin type-A receptor 8 antibody

HEK3 antibody

KIAA1459 antibody

Tyrosine-protein kinase receptor EEK antibody

Tyrosylprotein Kinase antibody

General Information

Product

Ephrin Receptor A3 Antibody

Description

Affinity Purified Ephrin Type-A Receptor 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 900-980 on human EPHA3 protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P29320

Overview

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Isoform 1:  Cell membrane; Single-pass type I membrane protein.  Isoform 2:  Secreted

Expression

Widely expressed. Highest level in placenta.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding.

Alternative Nomenclature

AW492086 antibody

Cek4 antibody

EC 2.7.10.1 antibody

EK4 antibody

End3 antibody

Eph receptor A3 antibody

EPH-like kinase 4 antibody

EPH-like tyrosine kinase 1 antibody

EphA3 antibody

Ephrin type-A receptor 3 antibody

ETK 1 antibody

ETK antibody

HEK 4 antibody

HEK antibody

Human embryo kinase 1 antibody

Mek4 antibody

MGC109882 antibody

Receptor tyrosine kinase HEK antibody

Tyro 4 antibody

Tyrosine-protein kinase TYRO4 antibody

General Information

Product

BACE2 A/C Antibody

Description

Affinity Purified Beta Secretase beta-site APP cleaving Enzyme 2A/C Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 490-518 on human BACE2 protein.

Accession #

GenBank: AAQ89286

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.72-0.76 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y5Z0

Overview

Responsible for the proteolytic processing of the amyloid precursor protein. Cleaves APP between 690 and 691 leading to the generation and extracellular release of beta-cleaved soluble APP and C-terminal fragment which is later released by gamma-secretase.

Molecular Function

Aspartic-type endopeptidase activity.

Subcellular Location

Endoplasmic reticulum, Endosome, Golgi apparatus; Membrane

Expression

Neurons within the hippocampus, frontal cortex and temporal cortex. Lower expression in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Also found in spinal cord, medulla oblongata, substania nigra and locus coruleus. Expressed in ductal epithelium of prostate.

Structure

Belongs to the peptidase A1 family.

Alternative Nomenclature

ALP 56 antibody

ASP1 antibody

ASP21 antibody

Aspartic Like Protease 56kDa antibody

Downs Syndrome Region Aspartic Protease antibody

DRAP antibody

Memapsin-1 antibody

Membrane associated aspartic protease 1 antibody

Theta-secretase antibody

General Information

Product

MACF1 Antibody

Description

Affinity Purified Microtuble-Actin crosslinking factor 1 Antibody

Verified Applications

ELISA, ICC, IFC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Recombinant protein

Accession #

GenBank: CAM18553.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9QXZO

Overview

F-actin bidning protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds microtubles.  Acts as positive regulator of Wnt receptor signaling pathway and is invovled in translocation of AXIN12 and its associated complex from the cytoplasm to cell membrane.  Has actin-regulated ATPase activity and is inovled in focal adhesios assembly and dynamics.

Molecular Function

Actin binding, calcium ion binding, ATPase activity, microtuble binding

Subcellular Location

Hair follicle stem cells; Ubiquitous expression; lung; heart; thymus; spleen; brain.

Expression

Expressed in neurontal tissues and foregut of embryo

Structure

Complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Alternative Nomenclature

ABP620 antibody

Acf7 antibody

Aclp7 antibody

Actin cross linking family 7 antibody

MACF antibody

Microtubule actin crosslinking factor 1 antibody

rabeculin alpha antibody

General Information

Product

Axin 2 Antibody

Description

Affinity Purified Axis Inhibition Protein 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 800-843 on human Axin 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y2T1

Overview

Inhibitor of Wnt signaling pathway.  Down-regulates beta-catenin.

Molecular Function

Developmental Protein; Wnt signaling pathway

Subcellular Location

Cytoplasm

Expression

Brain and lymphoblast

Structure

Contains 1 DIX domain and 1 RGS domain

Alternative Nomenclature

Axil antibody

Axin like protein antibody

Axis inhibition protein 2 antibody

Conductin antibody

General Information

Product

BACE2 B Antibody

Description

Affinity Purified beta-secretase 2 isoform B preproprotein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 350-396 on human BACE2B protein.

Accession #

NCBI:  NP_620477

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y5Z0

Overview

Responsible for the proteolytic processing of the amyloid precursor protein. Cleaves APP between 690 and 691 leading to the generation and extracellular release of beta-cleaved soluble APP and C-terminal fragment which is later released by gamma-secretase.

Molecular Function

Aspartic-type endopeptidase activity.

Subcellular Location

Endoplasmic reticulum, Endosome, Golgi apparatus; Membrane

Expression

Neurons within the hippocampus, frontal cortex and temporal cortex. Lower expression in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Also found in spinal cord, medulla oblongata, substania nigra and locus coruleus. Expressed in ductal epithelium of prostate.

Structure

Belongs to the peptidase A1 family.

Alternative Nomenclature

ALP 56 antibody

ASP1 antibody

ASP21 antibody

Aspartic Like Protease 56kDa antibody

Downs Syndrome Region Aspartic Protease antibody

DRAP antibody

Memapsin-1 antibody

Membrane associated aspartic protease 1 antibody

Theta-secretase antibody

General Information

Product

BMP11 Antibody

Description

Affinity Purified Bone morphogenetic protein 11 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-100 on human BMP11/GDF11 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.66 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O95390

Overview

Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development.

Molecular Function

Cytokine; Growth Factor; Neurogenesis

Subcellular Location

Secreted

Structure

Homodimer; Disulfide linked

Alternative Nomenclature

GDF 11 antibody

Growth differentiation factor 11 antibody

General Information

Product

Aph 1b Antibody

Description

Affinity Purified Presenelin-stabiliziation factor-like Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

C. elegans, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 235-257 of Human Presenilin stabilizing protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8WW43

Overview

Subunit of gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and beta-amyloid precursor protein.

Molecular Function

Notch signaling pathway

Subcellular Location

Membrane; Multi-pass protein

Expression

Weakly expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, skeletal muscle, heart, and brain.

Structure

Component of gamma-secretase complex.  Interacts with PSEN1 and PSEN2.

Alternative Nomenclature

Anterior pharynx defective 1B like antibody

Gamma secretase subunit APH 1B antibody

Presenilin stabilisation factor like antibody

PRO1328 antibody

PSFL antibody

TAAV 688 antibody