General Information

Product

DEK Antibody

Description

Affinity Purified DEK Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Predicted: Cat, Cow

Immunogen

Synthetic peptide taken within residues corresponding to a region within amino acids 324-373 of human DEK.

Region:

ELKETIKKLL ASANLEEVTM KQICKKVYEN YPTYDLTERK DFIKTTVKEL

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Dot blot

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Predicted molecular weight: 43 kDa

Protein

Uniprot #

P35659

Overview

Involved in chromatin organization

Molecular Function

Chromatin regulator; DNA-binding

Subcellular Location

Nucleus

Expression

Ubiquitous

Structure

Contains 1 SAP domain

Alternative Nomenclature

D6S231E antibody

General Information

Product

E2F2 Antibody Affinity Purified

Description

Affinity Purified Transcription Factor E2F2 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 70-120 on E2F2 protein.

Accession #

NCBI: NP_808401.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P56931

Overview

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication

Molecular Function

core promoter binding; sequence-specific DNA binding transcription factor activity.

Subcellular Location

Nucleus

Expression

developing epidermis and intestinal epithelium;thymus; liver;nervous system

Structure

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members

Alternative Nomenclature

dE2F2 antibody

General Information

Product

E2F3 Antibody

Description

Affinity Purified Transcription Factor E2F3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide taken within amino acid region 70-120 on E2F3 protein.

Accession #

GenBank: CAI24679.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.78-0.89 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O35261

Overview

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication

Molecular Function

core promoter binding; sequence-specific DNA binding transcription factor activity.

Subcellular Location

Nucleus

Expression

developing nervous system;spinal cord

Structure

Component of the DRTF1/E2F transcription factor complex

Alternative Nomenclature

DKFZp686C18211 antibody

KIAA0075 antibody

MGC104598 antibody

General Information

Product

E2F4 Antibody

Description

Affinity Purified Transcription Factor E2F4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 360-410 on mouse E2F4 protein.

Accession #

NCBI:  NP_683754.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.78-0.89 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8R0K9

Overview

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication

Molecular Function

DNA binding; sequence-specific DNA binding transcription factor activity.

Subcellular Location

Nucleus

Structure

Component of the DRTF1/E2F transcription factor complex

Alternative Nomenclature

E2F 4 antibody

E2F transcription factor 4 antibody

E2F transcription factor 4 p107/p130 binding antibody

p107/p130 binding protein antibody

Transcription factor E2F4 antibody

General Information

Product

E2F5 Antibody Affinity Purified

Description

Affinity Purified Transcription Factor E2F5 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 5-55 on E2F5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.88-0.96 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q61502

Overview

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation

Molecular Function

DNA binding; sequence-specific DNA binding transcription factor activity.

Subcellular Location

Nucleus

Expression

In the developing epidermis,nervous system;choroid plexus;kidney; liver; lung; heart

Structure

Component of the DRTF1/E2F transcription factor complex

Alternative Nomenclature

E2F 5 antibody

E2F transcription factor 5 antibody

E2F transcription factor 5 p130 binding antibody

p130 binding antibody

General Information

Product

FUBP1 Antibody

Description

Affinity Purified Far upstream-element binding protein 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-644 on human FUBP1 protein.

Accession #

GenBank: AAH17247.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.53-0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96AE4

Overview

Regulates the expression of MYC via binding to a single stranded far-upstream element upstream of the MYC promoter.  May act as both an activator and repressor of transcription.

Molecular Function

poly(A) RNA binding, single stranded DNA binding

Subcellular Location

Nucleus

Structure

Present in complex with PU560 and far upstream element DNA segment.  Interacts with PU560 and JTV1.

Alternative Nomenclature

DNA helicase V antibody

far upstream element (FUSE) binding protein 1 antibody

Far upstream element (FUSE) binding protein 4 antibody

FBP antibody

FUBP antibody

Fubp4 antibody

FUSE-binding protein 1 antibody

HDH V antibody

General Information

Product

DDIT3 Antibody

Description

Affinity Purified DNA damage-inducible transcript 3 protein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-160 on human GADD153 protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35638

Overview

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes.

Molecular Function

Activator, Repressor, DNA binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which blocks its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4.

Alternative Nomenclature

C/EBP homologous protein antibody

C/EBP zeta antibody

CCAAT/enhancer binding protein homologous protein antibody

CEBPZ antibody

CHOP 10 antibody

DDIT3 antibody

DNA Damage Inducible Transcript 3 antibody

GADD153 antibody

Growth Arrest and DNA Damage Inducible Protein 153 antibody

MGC4154 antibody

General Information

Product

HAND2 Antibody

Description

Affinity Purified heart- and neural crest derivatives-expressed protein 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on Human HAND2 protein.

Accession #

NCBI: NP_073187

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.5-.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P61295

Overview

Essential for cardiac morphogenesis, particularly for he formation of the right ventricle and of the aortic arch arteries.

Molecular Function

DNA-Binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AI225906 antibody

AI661148 antibody

autonomic nervous system and neural crest derivatives-expressed protein 2 antibody

Basic helix loop helix transcription factor HAND2 antibody

bHLHa26 antibody

Class A basic helix-loop-helix protein 26 antibody

Deciduum antibody

DHAND 2 antibody

Ehand 2 antibody

FLJ16260 antibody

heart antibody

Hed antibody

Highly similar to dHAND M musculus antibody

MGC125303 antibody

MGC125304 antibody

Th 2 antibody

Thing2 antibody

General Information

Product

IFI16 Antibody Affinity Purified

Description

Affinity Purified Gamma-interferon-inducible protein 16 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 140-190 on human IFI16 protein

Accession #

NCBI:  NP_001193496.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q16666

Overview

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein.

Molecular Function

Core promoter binding, identical protein binding, transcription factor binding, double-standard DNA binding, poly(A) RNA binding

Subcellular Location

Nucleus, cytoplasm (Note: Cellular distribution is dependent on acetylation status of multipartite nuclear localization signal(NLS); NLS acetylation promotes cytoplasmic localization. Localizes in nucleus during human herbes simplex virus 1 infection)

Expression

Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prosta

Alternative Nomenclature

IFI16 antibody

Gamma interferon inducible protein 16 antibody

Ifi204 antibody

IFNGIP1 antibody

interferon activated gene 204 antibody

Interferon gamma inducible protein 16 antibody

p204 antibody

PYHIN2 antibody

General Information

Product

KLF6 isoform C Antibody Affinity Purified

Description

Affinity Purified Guanine nucleotide binding protein (G protein), gamma 13a Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human KLF6 protein.

Accession #

NCBI: NP_001159597.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B3DIM4

Molecular Function

Signal transducer activity

Alternative Nomenclature

B cell derived protein 1 antibody

CBA1 antibody

COPEB antibody

GBF antibody

GC rich binding factor antibody

Klf6 antibody

Kruppel like zinc finger protein Zf9 antibody

PAC1 antibody

Proto-oncogene BCD1 antibody

ST12 antibody

Transcription factor Zf9 antibody

Zf9 antibody