General Information

Product

E-Cadherin Antibody

Description

Affinity Purified E-cadherin 1 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human E-cadherin 1 protein.

Accession #

GenBank: ADC53120.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3XNU5

Overview

Cadherins are calcium dependent cell adhesion proteins.

Molecular Function

Calcium ion binding;

Subcellular Location

Membrane; Single-pass type I  membrane protein

Structure

Repeat; Transmembrane helix

Alternative Nomenclature

Arc 1 antibody

Cadherin 1 antibody
cadherin 1 type 1 E-cadherin antibody
Cadherin1 antibody
CAM 120/80 antibody
CD324 antigen antibody
CDH1 antibody
CDHE antibody
E-Cad/CTF3 antibody
E-cadherin antibody
Epithelial cadherin antibody
epithelial calcium dependant adhesion protein antibody
LCAM antibody
Liver cell adhesion molecule antibody
UVO antibody
Uvomorulin antibody

General Information

Product

E-Cadherin Antibody

Description

Affinity Purified E-cadherin 1 Antibody Mid-Region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 350-400 human E-cadherin 1 protein.

Accession #

GenBank: ADC53120.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3XNU5

Overview

Cadherins are calcium dependent cell adhesion proteins.

Molecular Function

Calcium ion binding;

Subcellular Location

Membrane; Single-pass type I  membrane protein

Structure

Repeat; Transmembrane helix

Alternative Nomenclature

Arc 1 antibody

Cadherin 1 antibody
cadherin 1 type 1 E-cadherin antibody
Cadherin1 antibody
CAM 120/80 antibody
CD324 antigen antibody
CDH1 antibody
CDHE antibody
E-Cad/CTF3 antibody
E-cadherin antibody
Epithelial cadherin antibody
epithelial calcium dependant adhesion protein antibody
LCAM antibody
Liver cell adhesion molecule antibody
UVO antibody
Uvomorulin antibody

General Information

Product

E-Cadherin Antibody C-epitope

Description

Affinity Purified E-cadherin 1 Antibody

C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region  650-700 on human E-cadherin 1 protein.

Accession #

GenBank: ADC53120.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3XNU5

Overview

Cadherins are calcium dependent cell adhesion proteins.

Molecular Function

Calcium ion binding;

Subcellular Location

Membrane; Single-pass type I  membrane protein

Structure

Repeat; Transmembrane helix

Alternative Nomenclature

Arc 1 antibody

Cadherin 1 antibody
cadherin 1 type 1 E-cadherin antibody
Cadherin1 antibody
CAM 120/80 antibody
CD324 antigen antibody
CDH1 antibody
CDHE antibody
E-Cad/CTF3 antibody
E-cadherin antibody
Epithelial cadherin antibody
epithelial calcium dependant adhesion protein antibody
LCAM antibody
Liver cell adhesion molecule antibody
UVO antibody
Uvomorulin antibody

General Information

Product

beta Catenin Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 50-100 on human beta CTNNB protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P35222 

Overview

Major downstream component of the canonical Wnt signalling pathway.  Also involved in the regulation of cell adhesion and acts as a negative regulator of centrosome cohesion.

Molecular Function

Activator; Alpha-catenin binding; Cadherin binding; signal transducer activity

Subcellular Location

Cytoplasm; Nucleus; Cell junction; Cell Membrane

Expression

Expressed in hair follicle cell types including basal and peripheral matrix cells as well as cells of the outer and inner root sheaths.  Also expressed in colon and is present in cortical neurons at protein level.

Structure

Two separate complex associated pools found in cytoplasm, majority as compenent of an E-cadheren/catenin adhesion complex.  Another cytoplasmic pool is a part of a large complex which holds AXIN1, AXIN2, APC, CSNK1A1 and GSK3B

Alternative Nomenclature

Cadherin associated protein antibody

Catenin (cadherin associated protein), beta 1, 88kDa antibody

CATNB antibody

CHBCAT antibody

General Information

Product

beta Catenin Antibody Affinity Purified

Description

Affinity Purified Beta Catenin Antibody

C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 740-790 on human beta CTNNB protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P35222

Overview

Major downstream component of the canonical Wnt signalling pathway.  Also involved in the regulation of cell adhesion and acts as a negative regulator of centrosome cohesion.

Molecular Function

Activator; Alpha-catenin binding; Cadherin binding; signal transducer activity

Subcellular Location

Cytoplasm; Nucleus; Cell junction; Cell Membrane

Expression

Expressed in hair follicle cell types including basal and peripheral matrix cells as well as cells of the outer and inner root sheaths.  Also expressed in colon and is present in cortical neurons at protein level.

Structure

Two separate complex associated pools found in cytoplasm, majority as compenent of an E-cadheren/catenin adhesion complex.  Another cytoplasmic pool is a part of a large complex which holds AXIN1, AXIN2, APC, CSNK1A1 and GSK3B

Alternative Nomenclature

Cadherin associated protein antibody

Catenin (cadherin associated protein), beta 1, 88kDa antibody

CATNB antibody

CHBCAT antibody

General Information

Product

Syndecan-1 Antibody Affinity Purified

Description

Affinity Purified Syndecan 1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 250-350 on human Syndecan-1 protein.

Accession #

GenBank: AAH08765.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P18827

Overview

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Molecular Function

Protein C-terminus binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain).

Alternative Nomenclature

CD 138 antibody

Heparan sulfate proteoglycan fibroblast growth factor receptor antibody

SDC 1 antibody

SDC antibody

syndecan proteoglycan 1 antibody

General Information

Product

Syndecan-1 Antibody Affinity Purified

Description

Affinity Purified Syndecan 1 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 1-100 on human Syndecan-1 protein.

Accession #

GenBank: AAH08765.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P18827

Overview

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Molecular Function

Protein C-terminus binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain).

Alternative Nomenclature

CD 138 antibody

Heparan sulfate proteoglycan fibroblast growth factor receptor antibody

SDC 1 antibody

SDC antibody

syndecan proteoglycan 1 antibody

General Information

Product

ORP150 Antibody

Description

Affinity Purified Hypoxia up regulated 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 950-999 on human Hypoxia up regulated 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y4L1

Overview

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.

Molecular Function

ATP binding; chaperone binding

Subcellular Location

Endoplasmic reticulum lumen

Expression

Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.

Structure

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the heat shock protein 70 family.

Alternative Nomenclature

150 kDa oxygen regulated protein antibody

170 kDa glucose regulated protein antibody

DKFZp686N08236 antibody

FLJ94899 antibody

FLJ97572 antibody

glucose regulated protein 170 antibody

Grp 170 antibody

Grp170 antibody

HSP 12A antibody

HSP12A antibody

Hyou1 antibody

Hypoxia up regulated 1 antibody

Orp 150 antibody

Orp150 antibody

oxygen regulated protein (150kD) antibody

General Information

Product

VEGFR1 Antibody

Description

Affinity Purified Vascular Endothelial Growth Factor Receptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1250-1300 on human Vascular endothelial growth factor receptor 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P17948

Overview

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Molecular Function

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1 :Cell membrane; Single-pass type I membrane protein

Endosome Note: Autophosphorylation promotes ubiquitination and endocytosis.

Isoform 2 :Secreted 

Isoform 3 :Secreted

Isoform 4 :Secreted

Isoform 5 :Cytoplasm

Isoform 6 :Cytoplasm 

Isoform 7 :Cytoplasm 

Expression

Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).

Structure

The second and third Ig-like C2-type (immunoglobulin-like) domains are sufficient for VEGFA binding. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 7 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

Alternative Nomenclature

EC 2.7.10.1 

FLT 

FLT1 

Fms like tyrosine kinase 1 

FRT 

Soluble VEGF receptor 1 14 

Soluble VEGFR1 variant 2 

Soluble VEGFR1 variant 21 

Tyrosine protein kinase FRT 

Tyrosine protein kinase receptor FLT 

Vascular endothelial growth factor vascular permeability factor receptor 

Vascular permeability factor receptor 1 

VEGFR1

General Information

Product

E-Cadherin Antibody

Description

Affinity Purified E-cadherin 1 Antibody Multi-Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Multiple synthetic peptides taken within amino acid regions 50-100, 350-400, and 650-700 on human E-cadherin 1 protein.

Accession #

GenBank: ADC53120.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-Epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3XNU5

Overview

Cadherins are calcium dependent cell adhesion proteins.

Molecular Function

Calcium ion binding;

Subcellular Location

Membrane; Single-pass type I  membrane protein

Structure

Repeat; Transmembrane helix

Alternative Nomenclature

Arc 1 antibody

Cadherin 1 antibody

cadherin 1 type 1 E-cadherin antibody

CAM 120/80 antibody

CD 234 antibody

CD 324 antibody

CDH1 antibody

CDHE antibody

E-Cad/CTF3 antibody

E-cadherin antibody

ECAD antibody

Epithelial cadherin antibody

epithelial calcium dependant adhesion protein antibody

LCAM antibody

Liver cell adhesion molecule antibody

UVO antibody

Uvomorulin antibody