General Information

Product

Glucose Transporter GLUT1 Antibody

Description

Glucose transporter glycoprotein GLUT1 Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Sheep

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on GLUT1 protein.

Accession #

GenBank: AAC28635.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:12,000

ELISA

1:12,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:5,000

Protein

Uniprot #

P11166

Overview

Facilitative glucose transporter. May be responsible for constitutive or basal glucose uptake.

Molecular Function

Sugar Transport

Subcellular Location

Cell membrane; Multi-pass membrane protein; melanosome

Expression

Detected in erythrocytes. Variable expression in many human tissues.

Structure

Transmembrane helix

Alternative Nomenclature

DYT17 antibody

DYT18 antibody

erythrocyte/brain antibody

Erythrocyte/hepatoma glucose transporter antibody

facilitated glucose transporter member 1 antibody

Glucose transporter type 1 erythrocyte/brain antibody

GLUT1DS antibody

GLUTB antibody

GT1 antibody

GTG1 antibody

Gtg3 antibody

HepG2 glucose transporter antibody

MGC141895 antibody

MGC141896 antibody

PED antibody

RATGTG1 antibody

SLC2A1 antibody

Solute carrier family 2, facilitated glucose transporter member 1 antibody

General Information

Product

DOC2B Antibody

Description

Affinity Purified Double C2-like domain-containing protein beta Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic Peptide taken within amino acid region 360-412 on DOC2b gene.

Accession #

GenBank:  AAB47747.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P70610

Overview

calcium ion-dependent exocytosis of neurotransmitter;positive regulation of insulin secretion

Molecular Function

calcium-dependent phospholipid binding;transporter activity

Subcellular Location

Cell membrane, Cytoplasm, Membrane

Expression

Expressed in brain; highly enriched in neurons

Alternative Nomenclature

Double C2-like domain-containing protein beta antibody

OTTMUSP00000006522 antibody

RP23 78H4.2 antibody

General Information

Product

Insulin degrading enzyme Antibody

Verified Applications

ELISA, IP WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human Insulin-degrading enzyme protein.

Accession #

GenBank: EAW50092.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI00002289E9

Overview

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

Molecular Function

ATPase activity; Ubiquitin Binding; Zinc ion binding; Insulin binding; Hydrolase; Metalloprotease; Protease

Subcellular Location

Cytoplasm; Cell membrane; Secreted

Structure

Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus.

Alternative Nomenclature

Abeta-degrading protease antibody

FLJ35968 antibody

Ide antibody

insulin protease antibody

Insulin-degrading enzyme antibody

insulinase antibody

insulysin antibody

General Information

Product

Glucose-6-Phosphate, catalytic subunit Antibody Affinity Purified

Description

Affinity Purified Glucose-6-phosphoate catalytic 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 80-130 on G6PC2 protein, and common in larger variant of glucose 6 phosphatase.

Accession #

GenBank: CAM24718

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Z186

Overview

May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis.

Molecular Function

Gluconeogenesis

Subcellular Location

Endoplasmic Reticulum Membrane; Multi-pass membrane protein

Expression

Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart.

Structure

Belongs to the glucose-6-phosphatase family.

Alternative Nomenclature

AW107337 antibody

G-6-Pase antibody

g6pc antibody

G6PT antibody

Glucose-6-phosphatase alpha antibody

GSD1 antibody

GSD1a antibody

MGC163350 antibody

MGC93613 antibody

RP23-281C18.19 antibody

General Information

Product

GLP2R Antibody

Description

Affinity Purified Glucagon like peptide 2 Receptor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 375-425 on human GLP2R protein.

Accession #

GenBank: AAH43390.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q86XI5

Overview

GPCR activity

Subcellular Location

Cell membrane; Membrane

Alternative Nomenclature

GLP 2 R antibody

GLP 2 receptor antibody

GLP2R antibody

Glucagon-like peptide 2 receptor antibody

General Information

Product

P2Y1 Antibody

Description

Affinity Purified P2Y purinoceptor 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 360-410 on rat P2Y1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.85 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P49651

Overview

Serves as a receptor for extracellular adenine nucleotides, for example ATP and ADP.  It may mediate some effects of extracellular ATP on insulin secretion in the pancreatic islets.

Molecular Function

G-protein coupled receptor, Transducer, Receptor

Subcellular Location

Cell membrane; Multipass membrane protein

Expression

Is found to be expressed in muscle, heart, liver, kidney, lung, brain, spleen.  Not expressed in testis.

Alternative Nomenclature

ATP receptor antibody

P2 purinoceptor subtype Y1 antibody

P2RY1 antibody

P2RY1_HUMAN antibody

P2Y purinoceptor 1 antibody

P2Y1 antibody

P2Y1 purinoceptor antibody

Platelet ADP receptor antibody

Purinergic receptor antibody

Purinergic receptor P2Y G protein coupled 1 antibody

Purinergic receptor P2Y1 antibody

General Information

Product

GSK3 beta Antibody

Description

Affinity Purified Glycogen Synthase Kinase-3 beta Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human GSK3 beta protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P49841

Overview

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.

Molecular Function

Developmental protein, Kinase, Serine/threonine-protein kinase, Signal tranduction inhibitor, Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.

Structure

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Glycogen Synthase Kinase 3 Beta antibody

GSK 3 beta antibody

Serine/threonine-protein kinase GSK3B antibody

General Information

Product

MOTS-c Antibody

Description

Affinity Purified Mitochondrial-Derived Peptide MOTS-c Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Full-length peptide corresponding to amino acids 1-16 on MOTS-c.

Accession #

GenBank: AJM13597.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Protein

Uniprot #

A0A0C5B5G6

Alternative Nomenclature

Mitochondrial Dervied Peptide MOTS-c

MOTS

MOTS c

General Information

Product

Insulin Receptor Antibody

Description

Affinity Purified Insulin receptor alpha and beta Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 900-950 on human Insulin receptor, transcript variant 2 protein. Cross reactive with both Insulin receptor alpha and insulin receptor beta variants.

Accession #

NCBI:  NM_001079817.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P06213

Overview

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.

Structure

Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds.

Alternative Nomenclature

CD220 antibody

HHF5 antibody

HIR A antibody

INSR alpha antibody

Insulin receptor antibody

Insulin receptor subunit alpha antibody

IR antibody

HHF5 antibody

human insulin receptor antibody

Insulin receptor subunit beta antibody

IR 1 antibody

General Information

Product

Insulin Receptor Antibody

Description

Affinity Purified Insulin receptor alpha and beta Antibody Extreme C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 950-1000 on human Insulin receptor, transcript variant 2 protein.

Accession #

NCBI:  NM_001079817.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P06213

Overview

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.

Structure

Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds.

Alternative Nomenclature

CD220 antibody

HHF5 antibody

HIR A antibody

INSR alpha antibody

Insulin receptor antibody

Insulin receptor subunit alpha antibody

IR antibody

HHF5 antibody

human insulin receptor antibody

Insulin receptor subunit beta antibody

IR 1 antibody