General Information

Product

C-Myc Antibody

Description

Affinity Purified myc proto-oncogene protein Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human cMyc protein.

Accession #

NCBI:  NP_002458

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01106

Overview

Transcription factor that binds DNA in non-specific manner.  Also specifically recognizes the core sequence 5'CAC[GA]TG-3'.  Activates transcription of growth-related genes.

Molecular Function

Activator; Transcription regulation; DNA-binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Myc proto oncogene protein antibody

myc-related translation/localization regulatory factor antibody

MYCC antibody

Myelocytomatosis oncogene antibody

Niard antibody

Nird antibody

Oncogene Myc antibody

Proto-oncogene c-Myc antibody

Protooncogene homologous to myelocytomatosis virus antibody

RNCMYC antibody

Transcription factor p64 antibody

Transcriptional regulator Myc-A antibody

V-Myc avian myelocytomatosis viral oncogene homolog antibody

General Information

Product

C-Myc Antibody Affinity Purified

Description

Affinity Purified myc proto-oncogene protein Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 400-454 on human cMyc protein.

Accession #

NCBI:  NP_002458

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01106

Overview

Transcription factor that binds DNA in non-specific manner.  Also specifically recognizes the core sequence 5'CAC[GA]TG-3'.  Activates transcription of growth-related genes.

Molecular Function

Activator; Transcription regulation; DNA-binding

Subcellular Location

Nucleus

Structure

Contains 1 basic helix-loop-helix domain

Alternative Nomenclature

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Myc proto oncogene protein antibody

myc-related translation/localization regulatory factor antibody

MYCC antibody

Myelocytomatosis oncogene antibody

Niard antibody

Nird antibody

Oncogene Myc antibody

Proto-oncogene c-Myc antibody

Protooncogene homologous to myelocytomatosis virus antibody

RNCMYC antibody

Transcription factor p64 antibody

Transcriptional regulator Myc-A antibody

V-Myc avian myelocytomatosis viral oncogene homolog antibody

General Information

Product

Gastrin Antibody Affinity Purified

Description

Affinity Purified Gastrin Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Gastrin preprotein.

Accession #

NCBI:  NP_000796.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01350

Overview

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.

Molecular Function

Hormone Activity

Subcellular Location

Secreted

Expression

Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.

Structure

Belongs to the gastrin/cholecytosokinin family

Alternative Nomenclature

Component II antibody

G14 antibody

G17 antibody

G34 antibody

G52 antibody

G6 antibody

GAS antibody

GAST antibody

gastrin antibody

Gastrin-14 antibody

Gastrin-17 antibody

Gastrin-34 antibody

Gastrin-52 antibody

Gastrin-6 antibody

Gastrin-71 antibody

General Information

Product

Gastrin Antibody Affinity Purified

Description

Affinity Purified Gastrin Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human Gastrin preprotein.

Accession #

NCBI:  NP_000796.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:50-1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:50-1:200

Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P01350

Overview

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.

Molecular Function

Hormone Activity

Subcellular Location

Secreted

Expression

Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.

Structure

Belongs to the gastrin/cholecytosokinin family

Alternative Nomenclature

Component II antibody

G14 antibody

G17 antibody

G34 antibody

G52 antibody

G6 antibody

GAS antibody

GAST antibody

gastrin antibody

Gastrin-14 antibody

Gastrin-17 antibody

Gastrin-34 antibody

Gastrin-52 antibody

Gastrin-6 antibody

Gastrin-71 antibody

General Information

Product

Ki67 Antibody

Description

Affinity Purified Antigen KI-67 isoform 1 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 2950-3000 on human antigen Ki67 isoform 1.

Accession #

NCBI:  NP_002408.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P46013

Overview

required for maintaining cell proliferation

Molecular Function

poly(A) RNA binding;protein C-terminus binding;ATP binding

Subcellular Location

Chromosome, Nucleus

Expression

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Alternative Nomenclature

Antigen identified by monoclonal antibody Ki 67 antibody

KIA antibody

MKI67 antibody

Proliferation related Ki 67 antigen antibody

RP11-380J17.2 antibody

General Information

Product

Ki67 Antibody

Description

Affinity Purified Antigen KI-67 isoform 1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1250-1300 on human antigen Ki67 isoform 1.

Accession #

NCBI:  NP_002408.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P46013

Overview

required for maintaining cell proliferation

Molecular Function

poly(A) RNA binding; protein C-terminus binding; ATP binding

Subcellular Location

Chromosome, Nucleus

Expression

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Alternative Nomenclature

Antigen identified by monoclonal antibody Ki 67 antibody

KIA antibody

MKI67 antibody

Proliferation related Ki 67 antigen antibody

RP11-380J17.2 antibody

General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. Cytoplasm
Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.
Isoform A : Nucleus. Cytoplasm
Note: Mainly nuclear.
Isoform 4 : Mitochondrion outer membrane
mitochondrial outer membrane
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody

General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 320-370 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. Cytoplasm
Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.
Isoform A : Nucleus. Cytoplasm
Note: Mainly nuclear.
Isoform 4 : Mitochondrion outer membrane
mitochondrial outer membrane
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody

General Information

Product

PrLZ / TPD52 Antibody

Description

Affinity Purified Prostate leucine zipper isoform 5 Antibody Mid-Region

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 20-70 on human Prostate leucine zipper isoform 5 protein.

Accession #

NCBI:  NP_001020423.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P55327

Molecular Function

calcium ion binding, protein heterodimerization activity, protein homodimerization activity

Subcellular Location

cytoplasm, perinuclear region of cytoplasm, endoplasmic reticulum

Expression

Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver

Structure

Forms a homodimer or heterodimer with other members of the family. All isoforms interact with several 14-3-3 proteins.

Alternative Nomenclature

D52 antibody

Human 19.8 kDa protein mRNA, complete cds antibody

N8L antibody

PC 1 antibody

PC1/MRPS28 fusion protein antibody

PrLZ antibody

Prostate and colon associated protein antibody

Prostate leucine zipper antibody

Protein N8 antibody

TPD52 antibody

Tumor protein D52 antibody

General Information

Product

PrLZ / TPD52 Antibody

Description

Affinity Purified Prostate leucine zipper isoform 5 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 101-151 on human Prostate leucine zipper isoform 5 protein.

Accession #

NCBI:  NP_001020423.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P55327

Molecular Function

calcium ion binding, protein heterodimerization activity, protein homodimerization activity

Subcellular Location

cytoplasm, perinuclear region of cytoplasm, endoplasmic reticulum

Expression

Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.

Structure

Forms a homodimer or heterodimer with other members of the family. All isoforms interact with several 14-3-3 proteins.

Alternative Nomenclature

D52 antibody

Human 19.8 kDa protein mRNA, complete cds antibody

N8L antibody

PC 1 antibody

PC1/MRPS28 fusion protein antibody

PrLZ antibody

Prostate and colon associated protein antibody

Prostate leucine zipper antibody

Protein N8 antibody

TPD52 antibody

Tumor protein D52 antibody