PDE4D Antibody

Product no.: PD4-401AP

$320.00

General Information
Product	PDE4D Antibody
Description	Affinity Purified Phosphodiesterase 4D Antibody
Verified Applications	ELISA, IP, ICC/IF, IHC-P, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	C-terminal cyclic peptide unique to all PDED subtypes variants.
Specificity	This antibody labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, and PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9, OR PDE4C.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.57 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Apical cell membrane, Cytoplasm, Membrane
Expression	Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Structure	Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
Alternative Nomenclature	cAMP specific 3',5' cyclic phosphodiesterase 4D antibody DKFZp686M11213 antibody 5''-cyclic phosphodiesterase 4D antibody cAMP specific phosphodiesterase PDE4D antibody cAMP specific phosphodiesterase PDE4D6 antibody DPDE 3 antibody Dunce like phosphodiesterase E3 antibody FLJ97311 antibody HSPDE4D antibody PDE 43 antibody PDE4DN2 antibody Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody Drosophila antibody STRK1 antibody

Protein information supplied by UniProt

FabGennix PDE4D Antibody has been used in 39 Publications

Applications: Confocal microscopy, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western blot

Reactivity: Bovine, Human, Mouse, and Rat

Wang, Jianjie, Susan Bingaman, and Virginia H. Huxley. "Intrinsic sex-specific differences in microvascular endothelial cell phosphodiesterases." American Journal of Physiology-Heart and Circulatory Physiology 298.4 (2010): H1146-H1154.

PMID: 20139324

Applications: Immunofluorescence, Western Blot

Reactivity: Rat

Massimi, Mara, et al. "Increase of Intracellular Cyclic AMP by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival." Journal of Cellular Biochemistry (2016).

PMID: 27859531

Applications: Western Blot

Reactivity: Human

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015).

PMID: 25578602

Applications: Immunohistochemistry

Reactivity: Human

Guo, Jiejie, et al. "Etazolate abrogates the lipopolysaccharide (LPS)-induced downregulation of the cAMP/pCREB/BDNF signaling, neuroinflammatory response and depressive-like behavior in mice." Neuroscience 263 (2014).

PMID: 24434771

Applications: Western Blot

Reactivity: Mouse

Dong, Cun-Jian, et al. "Presynaptic Inhibition by α2 Receptor/Adenylate Cyclase/PDE4 Complex at Retinal Rod Bipolar Synapse." The Journal of Neuroscience 34.28 (2014).

PMID: 25009274

Applications: Immunohistochemistry

Reactivity: Rat

Zhai, Kui, et al. "Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms." Cellular signalling 26.5 (2014).

PMID: 24463006

Applications: Western Blot

Reactivity: Rat

Salazar, Norma C et al. “GRK2 Blockade with βARKct Is Essential for Cardiac Β2-Adrenergic Receptor Signaling Towards Increased Contractility.” Cell Communication and Signaling : CCS 11 (2013).

PMCID: 3846709

Applications: Immunoprecipitation, Western Blot

Reactivity: Mouse

Al-Tawashi, Azza, and Chris Gehring. “Phosphodiesterase Activity Is Regulated by CC2D1A That Is Implicated in Non-Syndromic Intellectual Disability.” Cell Communication and Signaling?: CCS 11 (2013).

PMCID: 3704924

Applications: Immunocytochemistry, Western Blot

Reactivity: Mouse

Wang, Zhen-Zhen et al. “RNA Interference-Mediated Phosphodiesterase 4D Splice Variants Knock-down in the Prefrontal Cortex Produces Antidepressant-Like and Cognition-Enhancing Effects.” British Journal of Pharmacology 168.4 (2013).

PMCID: 3631387

Applications: Western Blot

Reactivity: Mouse

Michalski, Joel, et al. "Attenuation of inhibitory prostaglandin E2 signaling in Human lung fibroblasts is mediated by phosphodiesterase 4." American journal of respiratory cell and molecular biology 47.6 (2012).

PMID: 23043089

Applications: Western Blot

Reactivity: Human

Al-Tawashi, Azza et al. “Protein Implicated in Nonsyndromic Mental Retardation Regulates Protein Kinase A (PKA) Activity.” The Journal of Biological Chemistry 287.18 (2012).

PMCID: 3340277

Applications: Immunohistochemistry, Western Blot

Reactivity: Mouse

Click Here for the complete list of all FabGennix PDE4D Antibody publications

Publishing research using PD4-401AP? Please let us know, we can cite the reference in this datasheet.

To view the PDE4D Antibody specification sheet, click the link below:

FabGennix PD4-401AP Specifcation Sheet

Customer ratings for PDE4D Antibody

Number of ratings: 1
Average rating: 5

Excellent
from Verified Customer on 12/14/2020

Immunocytochemistry/Immunofluorescence in mouse heart. No Permeabilization. Serum as blocking agent for 1 hour at 10%. Formaldehyde fixed. Incubated for 16 hours at 16C.

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Accessories

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Note

Status

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PDE4D Antibody FITC

PD4D-FITC

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PDE4D Antibody BIOTIN

PD4D-BIOTIN

$375.00 *

PDE4D Blocking Peptide

P-PD4D

$155.00 *

PDE4D Positive Control

PC-PD4D

$220.00 *

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