General Information

Product

Phospho-JAK2 Antibody

Description

Affinity Purified Phosphorylated Tyrosine-protein kinase JAK2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human JAK2 protein. Phosphorylated 1008 & 1009 tyrosine residues.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O60674

Overview

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor.

Molecular Function

Adaptive immunity, Immunity, Innate immunity

Subcellular Location

Endomembrane system; Peripheral membrane protein; Cytoplasm; Nucleus

Expression

Ubiquitously expressed throughout most tissues.

Structure

Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1.

Alternative Nomenclature

JAK 2 

Janus Activating Kinase 2 

Janus kinase 2 (a protein tyrosine kinase) 

Janus kinase 2 

JTK 10 

THCYT3 

Tyrosine protein kinase JAK2 

General Information

Product

PRAS40 Antibody

Description

Affinity Purified Proline-rich AKT1 substrate 1 isoform a Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein.

Accession #

NCBI:  NP_115751

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96B36

Overview

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Molecular Function

negative regulation of cell size, negative regulation of protein kinase activity, negative regulation of TOR signaling, neurotrophin TRK receptor signaling pathway, regulation of cellular response to heat, regulation of neuron apoptotic process

Subcellular Location

Cytoplasm > cytosol

Expression

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Alternative Nomenclature

40 kDa proline rich AKT substrate antibody
AKT1 S1 antibody
AKT1 substrate 1 (proline rich) antibody
AKT1S1 antibody
Lobe antibody
PRAS antibody
PRAS40 antibody
Proline rich Akt substrate 40 kDa antibody
Proline-rich AKT1 substrate 1 antibody

General Information

Product

Aurora A Antibody

Description

Affinity Purified Aurora Kinase A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-397 on human Aurora Kinase A protein.

Accession #

NCBI:  NP_695208

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q3MHU2

Overview

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes.

Molecular Function

ATP binding

histone serine kinase activity

protein heterodimerization activity

protein kinase activity

protein kinase binding

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

ubiquitin protein ligase binding

Subcellular Location

centrosome 

spindle pole 

cilium basal body

centriole

Expression

Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Alternative Nomenclature

AIK antibody

ARK1 antibody

AURA antibody

Aurora 2 antibody

Aurora A antibody

Aurora kinase A antibody

Aurora-related kinase 1 antibody

Aurora/IPL1 like kinase antibody

AURORA2 antibody

Breast tumor-amplified kinase antibody

BTAK antibody

hARK1 antibody

IAK antibody

IPL1 related kinase antibody

PPP1R47 antibody

Protein phosphatase 1, regulatory subunit 47 antibody

Serine/threonine kinase 15 antibody

Serine/threonine kinase 6 antibody

Serine/threonine-protein kinase aurora-A antibody

STK15 antibody

STK6 antibody

STK7 antibody

General Information

Product

Aurora B Antibody

Description

Aurora Kinase B Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 300-344 on human Aurora Kinase B protein.

Accession #

NCBI:  NP_004208.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q96GD4

Overview

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.

Molecular Function

ATP binding

histone serine kinase activity

kinase binding

metal ion binding

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

Subcellular Location

Spindle, Nucleus

Expression

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Alternative Nomenclature

AIK2 antibody

AIM1 antibody

ARK2 antibody

AurB antibody

AURKB antibody

Aurora 1 antibody

Aurora and Ipl1 like midbody associated protein 1 antibody

Aurora kinase B antibody

Aurora related kinase 2 antibody

Aurora- and Ipl1-like midbody-associated protein 1 antibody

Aurora-B antibody

Aurora/IPL1 related kinase 2 antibody

IPL1 antibody

PPP1R48 antibody

Protein phosphatase 1 regulatory subunit 48 antibody

Serine/threonine-protein kinase 12 antibody

Serine/threonine-protein kinase aurora-B antibody

STK1 antibody

STK12 antibody

STK5 antibody

General Information

Product

WEE2 Antibody

Description

Affinity Purified Wee1-like protein kinase isoform 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-110 on mouse Wee1-like protein kinase isoform 2.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polylclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q66JT0

Molecular Function

ATP binding

magnesium ion binding

non-membrane spanning protein tyrosine kinase activity

protein tyrosine kinase activity

Subcellular Location

Nucleus, Cytoplasm

Expression

Ovary-specific.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Alternative Nomenclature

WEE1 homolog 2 (S. pombe) antibody

Wee1-like protein kinase 1B antibody

Wee1-like protein kinase 2 antibody

WEE1B antibody

Wee1B kinase antibody

Wee2 antibody

WEE2_HUMAN antibody

General Information

Product

PP2A-alpha Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A , B56- Alpha subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 410-486 on human Serine / Threonine-Protein Phosphatase 2A , B56- Alpha subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q15172

Overview

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Molecular Function

kinase binding

phosphoprotein phosphatase activity

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Centromere, Chromosome, Cytoplasm, Nucleus

Expression

Widely expressed with the highest expression in heart and skeletal muscle.

Structure

Belongs to the phosphatase 2A regulatory subunit B56 family.

Alternative Nomenclature

PP2A alpha antibody
PP2AA_HUMAN antibody
PP2Aalpha antibody
PP2Ac antibody
PP2CA antibody
PP2Calpha antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody
Protein phosphatase 2 catalytic subunit alpha isoform antibody
Protein phosphatase 2, catalytic subunit, alpha isozyme antibody
Protein phosphatase 2A catalytic subunit, alpha isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

General Information

Product

PP2A-beta Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A 56 kDa regulatory Beta subunit Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-497 on human Serine / Threonine-Protein Phosphatase 2A 56 kDa regulatory Beta subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q15173

Molecular Function

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Cytoplasm

Expression

Highest expression in brain.

Alternative Nomenclature

PP2A beta antibody
PP2AA_HUMAN antibody
PP2Abeta antibody
PP2Ac antibody
PP2CA antibody
PP2Cbeta antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit beta isoform antibody
Protein phosphatase 2 catalytic subunit beta isoform antibody
Protein phosphatase 2, catalytic subunit, beta isozyme antibody
Protein phosphatase 2A catalytic subunit, beta isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody

General Information

Product

PP2A-epsilon Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A 56 kDa regulatory Epsilon subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-467 on human serine / Threonine-Protein Phosphatase 2A 56 kDa regulatory Epsilon subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q16537

Overview

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Molecular Function

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Cytoplasm

Structure

Belongs to the phosphatase 2A regulatory subunit B56 family.

Alternative Nomenclature

PP2A epsilon antibody
PP2AA_HUMAN antibody
PP2Aepsilon antibody
PP2Ac antibody
PP2CA antibody
PP2Cepsilon antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit epsilon isoform antibody
Protein phosphatase 2 catalytic subunit epsilon isoform antibody
Protein phosphatase 2, catalytic subunit, epsilon isozyme antibody
Protein phosphatase 2A catalytic subunit, epsilon isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit epsilon isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit epsilon isoform antibody

General Information

Product

PP2A-delta Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A B56-Delta subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 540-600 on human Serine / Threonine-Protein Phosphatase 2A B56-Delta subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q14738

Overview

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Molecular Function

phosphoprotein phosphatase activity

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Cytoplasm, Nucleus

Expression

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

Structure

Belongs to the phosphatase 2A regulatory subunit B56 family.

Alternative Nomenclature

PP2A delta antibody
PP2AA_HUMAN antibody
PP2Adelta antibody
PP2Ac antibody
PP2CA antibody
PP2Cdelta antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit delta isoform antibody
Protein phosphatase 2 catalytic subunit delta isoform antibody
Protein phosphatase 2, catalytic subunit, delta isozyme antibody
Protein phosphatase 2A catalytic subunit, delta isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit delta isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit delta isoform antibody

General Information

Product

PP2A-gamma Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A B56 Gamma1 subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 390-439 on human Serine / Threonine-Protein Phosphatase 2A B56 Gamma1 subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q13362

Molecular Function

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Centromere, Chromosome, Nucleus

Expression

Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.

Structure

Belongs to the phosphatase 2A regulatory subunit B56 family.

Alternative Nomenclature

PP2A gamma antibody
PP2AA_HUMAN antibody
PP2Agamma antibody
PP2Ac antibody
PP2CA antibody
PP2Cgamma antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit gamma isoform antibody
Protein phosphatase 2 catalytic subunit gamma isoform antibody
Protein phosphatase 2, catalytic subunit, gamma isozyme antibody
Protein phosphatase 2A catalytic subunit, gamma isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit gamma isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit gamma isoform antibody